LPS- and LTA-induced expression of IL-6 and TNF-α in neonatal and adult blood

• Verfasst von: Koch, Lutz [VerfasserIn]
• Verfasst von: Frommhold, David [VerfasserIn]
• Verfasst von: Buschmann-Prayon, Kirsten [VerfasserIn]
• Verfasst von: Kuß, Navina [VerfasserIn]
• Verfasst von: Pöschl, Johannes [VerfasserIn]
• Verfasst von: Ruef, Peter [VerfasserIn]
• Titel: LPS- and LTA-induced expression of IL-6 and TNF-α in neonatal and adult blood
• Titelzusatz: role of MAPKs and NF-κB
• Verf.angabe: Lutz Koch, David Frommhold, Kirsten Buschmann, Navina Kuss, Johannes Poeschl, and Peter Ruef
• E-Jahr: 2014
• Jahr: 30 October 2014
• Umfang: 9 S.
• Fussnoten: Gesehen am 30.10.2020
• Titel Quelle: Enthalten in: Mediators of inflammation
• Ort Quelle: Sylvania, Ohio : Hindawi Publishing Corp., 1992
• Jahr Quelle: 2014
• Band/Heft Quelle: (2014) Artikel-Nummer 283126, 8 Seiten
• ISSN Quelle: 1466-1861
• DOI: doi:10.1155/2014/283126
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1737431211

Abstract

As nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) seem to be critical mediators in the inflammatory response, we studied the effects of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) on (a) the activation of NF-κB and MAPKs and (b) the expression of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) with or without the specific inhibitors of these intracellular signal transduction pathways in neonatal cord and adult blood. TNF-α and IL-6 concentrations showed a sharp increase in the supernatants of cord and adult whole blood after stimulation. TNF-α concentrations were significantly higher, whereas IL-6 concentrations were tendentially lower in adult blood after stimulation. Stimulation with LPS or LTA resulted in a significantly decreased activation of p38 MAPK in neonatal compared with adult blood. Although LTA failed to induce additional ERK1/2 phosphorylation, LPS stimulation mediated the moderately increased levels of activated ERK1/2 in neonatal monocytes. The addition of the p38 MAPK inhibitor SB202190 significantly decreased IL-6 and TNF-α production upon LPS or LTA stimulation. Furthermore, the inhibition of ERK1/2 was able to reduce LPS-stimulated TNF-α production in neonatal blood. We conclude that p38 MAPK as well as ERK1/2 phosphorylation is crucially involved in LPS activation and could explain the differences in early cytokine response between neonatal and adult blood.