Optogenetic control of nuclear protein export

• Verfasst von: Niopek, Dominik [VerfasserIn]
• Verfasst von: Wehler, Pierre [VerfasserIn]
• Verfasst von: Rönsch, Julia [VerfasserIn]
• Verfasst von: Eils, Roland [VerfasserIn]
• Verfasst von: Di Ventura, Barbara [VerfasserIn]
• Titel: Optogenetic control of nuclear protein export
• Verf.angabe: Dominik Niopek, Pierre Wehler, Julia Roensch, Roland Eils & Barbara Di Ventura
• E-Jahr: 2016
• Jahr: 8 Feb 2016
• Umfang: 9 S.
• Fussnoten: Gesehen am 03.11.2020
• Titel Quelle: Enthalten in: Nature Communications
• Ort Quelle: [London] : Nature Publishing Group UK, 2010
• Jahr Quelle: 2016
• Band/Heft Quelle: 7(2016), Artikel-ID 10624, Seite 1-9
• ISSN Quelle: 2041-1723
• DOI: doi:10.1038/ncomms10624
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1737650312

Abstract

Active nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present a light-inducible nuclear export system (LEXY) based on a single, genetically encoded tag, which enables precise spatiotemporal control over the export of tagged proteins. A constitutively nuclear, chromatin-anchored LEXY variant expands the method towards light inhibition of endogenous protein export by sequestering cellular CRM1 receptors. We showcase the utility of LEXY for cell biology applications by regulating a synthetic repressor as well as human p53 transcriptional activity with light. LEXY is a powerful addition to the optogenetic toolbox, allowing various novel applications in synthetic and cell biology.