Vitamin D effects on osteoblastic differentiation of mesenchymal stem cells from dental tissues

• Verfasst von: Posa, Francesca [VerfasserIn]
• Verfasst von: Di Benedetto, Adriana [VerfasserIn]
• Verfasst von: Colaianni, Graziana [VerfasserIn]
• Verfasst von: Cavalcanti-Adam, Elisabetta A. [VerfasserIn]
• Verfasst von: Brunetti, Giacomina [VerfasserIn]
• Verfasst von: Porro, Chiara [VerfasserIn]
• Verfasst von: Trotta, Teresa [VerfasserIn]
• Verfasst von: Grano, Maria [VerfasserIn]
• Verfasst von: Mori, Giorgio [VerfasserIn]
• Titel: Vitamin D effects on osteoblastic differentiation of mesenchymal stem cells from dental tissues
• Verf.angabe: Francesca Posa, Adriana Di Benedetto, Graziana Colaianni, Elisabetta A. Cavalcanti-Adam, Giacomina Brunetti, Chiara Porro, Teresa Trotta, Maria Grano, and Giorgio Mori
• Jahr: 2016
• Umfang: 9 S.
• Fussnoten: Gesehen am 03.11.2020
• Titel Quelle: Enthalten in: Stem cells international
• Ort Quelle: London [u.a.] : Sage-Hindawi, 2010
• Jahr Quelle: 2016
• Band/Heft Quelle: (2016), Artikel-Nummer 9150819, 9 Seiten
• ISSN Quelle: 1687-9678
• DOI: doi:10.1155/2016/9150819
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 173769221X

Abstract

1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of human Mesenchymal Stem Cells (hMSCs) from bone marrow. MSCs can be also isolated from the immature form of the tooth, the dental bud: Dental Bud Stem Cells (DBSCs) are adult stem cells that can effectively undergo osteoblastic differentiation. In this work we investigated the effect of Vit D on DBSCs differentiation into osteoblasts. Our data demonstrate that DBSCs, cultured in an opportune osteogenic medium, differentiate into osteoblast-like cells; Vit D treatment stimulates their osteoblastic features, increasing the expression of typical markers of osteoblastogenesis like RUNX2 and Collagen I (Coll I) and, in a more important way, determining a higher production of mineralized matrix nodules.