MAP1S controls microtubule stability throughout the cell cycle in human cells

• Verfasst von: Tegha Dunghu, Justus [VerfasserIn]
• Verfasst von: Bausch, Elena [VerfasserIn]
• Verfasst von: Ellenberg, Jan [VerfasserIn]
• Verfasst von: Gruss, Oliver [VerfasserIn]
• Titel: MAP1S controls microtubule stability throughout the cell cycle in human cells
• Verf.angabe: Justus Tegha-Dunghu, Elena Bausch, Beate Neumann, Annelie Wuensche, Thomas Walter, Jan Ellenberg and Oliver J. Gruss
• E-Jahr: 2014
• Jahr: November 30, 2014
• Umfang: 6 S.
• Fussnoten: Gesehen am 06.11.2020
• Titel Quelle: Enthalten in: Journal of cell science
• Ort Quelle: Cambridge : Company of Biologists Limited, 1853
• Jahr Quelle: 2014
• Band/Heft Quelle: 127(2014), 23, Seite 5007-5013
• ISSN Quelle: 1477-9137
• DOI: doi:10.1242/jcs.136457
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 173805120X

Abstract

Understanding the molecular basis for proper cell division requires a detailed functional analysis of microtubule (MT)-associated proteins. MT-associated protein 1S (MAP1S), the most ubiquitously expressed MAP1 family member, is required for accurate cell division. Here, using quantitative analysis of MT plus-end tracking, we show that MAP1S knockdown alters MT dynamics throughout the cell cycle. Surprisingly, MAP1S downregulation results in faster growing, yet short-lived, MTs in all cell cycle stages and in a global loss of MT acetylation. These aberrations correlate with severe defects in the final stages of cell division. In monopolar cytokinesis assays, we demonstrate that MAP1S guides MT-dependent initiation of cytokinesis. Our data underline the key role of MAP1S as a global regulator of MT stability and demonstrate a new primary function of MAP1S to regulate MT dynamics at the onset of cytokinesis.