| |  Online-Ressource |
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| Verfasst von: | Wandernoth, Petra Melitta [VerfasserIn]  |
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| | Kriegsmann, Katharina [VerfasserIn] |
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| | Groh-Mohanu, Cristina [VerfasserIn] |
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| | Daeumer, Martin [VerfasserIn] |
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| | Gohl, Peter [VerfasserIn] |
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| | Harzer, Oliver [VerfasserIn] |
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| | Kriegsmann, Mark [VerfasserIn] |
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| | Kriegsmann, Joerg [VerfasserIn] |
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| Titel: | Detection of severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) by mass spectrometry |
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| Verf.angabe: | Petra Wandernoth, Katharina Kriegsmann, Cristina Groh-Mohanu, Martin Daeumer, Peter Gohl, Oliver Harzer, Mark Kriegsmann and Joerg Kriegsmann |
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| E-Jahr: | 2020 |
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| Jahr: | 4 August 2020 |
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| Fussnoten: | Gesehen am 09.11.2020 |
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| Titel Quelle: | Enthalten in: Viruses |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 12(2020,8) Artikel-Nummer 849, 8 Seiten |
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| ISSN Quelle: | 1999-4915 |
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| Abstract: | Background: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS). Methods: Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared. Results: Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays. Conclusions: MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs. |
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| DOI: | doi:10.3390/v12080849 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/v12080849 |
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| | Volltext: https://www.mdpi.com/1999-4915/12/8/849 |
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| | DOI: https://doi.org/10.3390/v12080849 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | COVID19 |
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| | mass spectrometry |
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| | SARS-CoV2 |
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| | severe acute respiratory syndrome coronavirus 2 |
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| K10plus-PPN: | 1738229009 |
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| Verknüpfungen: | → Zeitschrift |
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Detection of severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) by mass spectrometry / Wandernoth, Petra Melitta [VerfasserIn]; 4 August 2020 (Online-Ressource)
