Modification of purified proteins with photochemical protection compounds for high-resolution photoactivation of protein function in vitro and in vivo

• Verfasst von: Cambridge, Sidney [VerfasserIn]
• Titel: Modification of purified proteins with photochemical protection compounds for high-resolution photoactivation of protein function in vitro and in vivo
• Verf.angabe: Sidney Cambridge
• E-Jahr: 2014
• Jahr: 21 March 2014
• Umfang: 12 S.
• Fussnoten: Gesehen am 10.11.2020
• Titel Quelle: Enthalten in: Photoswitching Proteins
• Ort Quelle: New York, NY : Humana Press, 2014
• Jahr Quelle: 2014
• Band/Heft Quelle: (2014), Seite 19-30
• ISBN Quelle: 978-1-4939-0470-9
• DOI: doi:10.1007/978-1-4939-0470-9_2
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cells, Cultured
• Sach-SW: Photolysis
• Sach-SW: Proteins
• Sach-SW: Radiation-Protective Agents
• K10plus-PPN: 1738291138

Abstract

Specific and targeted photoactivation of protein function inside cells, tissues, or whole organisms can be achieved with reversible inhibition of proteins by conjugation with photolabile protection compounds ("caging"). In vitro caging of proteins is thought to cause sterical or functional hindrance of amino acid side chains that are important for protein activity. Following the modification, the caged protein is introduced into the biological system and high-resolution irradiation ensures specific release of protein function in the desired areas. Here, I describe the entire caging procedure and highlight a few of the caveats of photoactivation in living cells.