| |  Online-Ressource |
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| Verfasst von: | McDowell, Melanie [VerfasserIn]  |
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| | Heimes, Michael [VerfasserIn] |
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| | Fiorentino, Francesco [VerfasserIn] |
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| | Mehmood, Shahid [VerfasserIn] |
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| | Farkas, Ákos [VerfasserIn] |
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| | Coy-Vergara, Javier [VerfasserIn] |
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| | Wu, Di [VerfasserIn] |
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| | Bolla, Jani Reddy [VerfasserIn] |
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| | Schmid, Volker [VerfasserIn] |
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| | Heinze, Roger J. [VerfasserIn] |
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| | Wild, Klemens [VerfasserIn] |
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| | Flemming, Dirk [VerfasserIn] |
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| | Pfeffer, Stefan [VerfasserIn] |
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| | Schwappach, Blanche [VerfasserIn] |
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| | Robinson, Carol V. [VerfasserIn] |
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| | Sinning, Irmgard [VerfasserIn] |
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| Titel: | Structural basis of tail-anchored membrane protein biogenesis by the GET insertase complex |
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| Verf.angabe: | Melanie A. McDowell, Michael Heimes, Francesco Fiorentino, Shahid Mehmood, Ákos Farkas, Javier Coy-Vergara, Di Wu, Jani Reddy Bolla, Volker Schmid, Roger Heinze, Klemens Wild, Dirk Flemming, Stefan Pfeffer, Blanche Schwappach, Carol V. Robinson, and Irmgard Sinning |
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| E-Jahr: | 2020 |
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| Jahr: | 9 September 2020 |
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| Umfang: | 22 S. |
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| Fussnoten: | Gesehen am 10.11.2020 |
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| Titel Quelle: | Enthalten in: Molecular cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 80(2020), 1, Seite 72-86.e7 |
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| ISSN Quelle: | 1097-4164 |
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| Abstract: | Membrane protein biogenesis faces the challenge of chaperoning hydrophobic transmembrane helices for faithful membrane insertion. The guided entry of tail-anchored proteins (GET) pathway targets and inserts tail-anchored (TA) proteins into the endoplasmic reticulum (ER) membrane with an insertase (yeast Get1/Get2 or mammalian WRB/CAML) that captures the TA from a cytoplasmic chaperone (Get3 or TRC40, respectively). Here, we present cryo-electron microscopy reconstructions, native mass spectrometry, and structure-based mutagenesis of human WRB/CAML/TRC40 and yeast Get1/Get2/Get3 complexes. Get3 binding to the membrane insertase supports heterotetramer formation, and phosphatidylinositol binding at the heterotetramer interface stabilizes the insertase for efficient TA insertion in vivo. We identify a Get2/CAML cytoplasmic helix that forms a “gating” interaction with Get3/TRC40 important for TA insertion. Structural homology with YidC and the ER membrane protein complex (EMC) implicates an evolutionarily conserved insertion mechanism for divergent substrates utilizing a hydrophilic groove. Thus, we provide a detailed structural and mechanistic framework to understand TA membrane insertion. |
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| DOI: | doi:10.1016/j.molcel.2020.08.012 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.molcel.2020.08.012 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S109727652030575X |
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| | DOI: https://doi.org/10.1016/j.molcel.2020.08.012 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cryo-EM |
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| | EMC |
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| | GET/TRC pathway |
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| | lipid binding |
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| | membrane proteins |
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| | native mass spectrometry |
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| | protein transport |
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| | tail anchor |
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| | YidC |
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| K10plus-PPN: | 1738316653 |
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| Verknüpfungen: | → Zeitschrift |
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Structural basis of tail-anchored membrane protein biogenesis by the GET insertase complex / McDowell, Melanie [VerfasserIn]; 9 September 2020 (Online-Ressource)
