Segregation of effector mechanisms in a tumour-specific CD8+ t-cell clone correlates with CD30 expression

• Verfasst von: Sun, Yuansheng [VerfasserIn]
• Verfasst von: Song, Mingxia [VerfasserIn]
• Verfasst von: Schadendorf, Dirk [VerfasserIn]
• Titel: Segregation of effector mechanisms in a tumour-specific CD8+ t-cell clone correlates with CD30 expression
• Verf.angabe: Y. Sun, M. Song, M.J. Maeurer & D. Schadendorf
• E-Jahr: 2001
• Jahr: 23 December 2001
• Umfang: 7 S.
• Fussnoten: Gesehen am 10.11.2020
• Titel Quelle: Enthalten in: Scandinavian journal of immunology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1972
• Jahr Quelle: 2001
• Band/Heft Quelle: 54(2001), 3, Seite 314-320
• ISSN Quelle: 1365-3083
• DOI: doi:10.1046/j.1365-3083.2001.00971.x
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1738321169

Abstract

In this study we have analyzed CD30-antigen expression in three melanoma-directed cytotoxic T lymphocyte (CTL) clones with a T helper 0 (Th0)-like cytokine secretion profile (i.e. interleukin (IL)-4, IL-5, and interferon (IFN)-γ). We show that all CTL clones expressed high levels of CD30 upon contact with the autologous tumour cells. One CTL clone, termed A2 with a monoclonal feature was selected for further analyses and found its CD30 expression dependent on the presence of IL-4. Functionally, a CD30-expressing A2 CTL was capable of producing higher amounts of IFN-γ (up to 1.5-fold) and IL-4 (up to two-fold) than its CD30− counterpart. Furthermore, CD30-positive A2 CTL displayed an at least three-fold greater proliferative response to the tumour cell stimulation, contrasting with CD30− CTL. However, the antitumour cytotoxic activity of A2 CTL was not modulated by the CD30 expression. These results suggest that CD30 antigen can be inducible on a subset of tumour-directed CD8+ CTL, and that this subset of cells may have profound effector functions, such as cytokine secretion, proliferation, and cytotoxicity.