| |  Online-Ressource |
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| Verfasst von: | Bartosova, Maria [VerfasserIn]  |
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| | Herzog, Rebecca [VerfasserIn] |
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| | Ridinger, David [VerfasserIn] |
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| | Levai, Eszter [VerfasserIn] |
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| | Jenei, Hanna [VerfasserIn] |
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| | Zhang, Conghui [VerfasserIn] |
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| | González Mateo, Guadalupe T. [VerfasserIn] |
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| | Marinovic, Iva [VerfasserIn] |
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| | Hackert, Thilo [VerfasserIn] |
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| | Bestvater, Felix [VerfasserIn] |
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| | Hausmann, Michael [VerfasserIn] |
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| | López Cabrera, Manuel [VerfasserIn] |
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| | Kratochwill, Klaus [VerfasserIn] |
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| | Zarogiannis, Sotirios G. [VerfasserIn] |
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| | Schmitt, Claus P. [VerfasserIn] |
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| Titel: | Alanyl-glutamine restores tight junction organization after disruption by a conventional peritoneal dialysis fluid |
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| Verf.angabe: | Maria Bartosova, Rebecca Herzog, David Ridinger, Eszter Levai, Hanna Jenei, Conghui Zhang, Guadalupe T. González Mateo, Iva Marinovic, Thilo Hackert, Felix Bestvater, Michael Hausmann, Manuel López Cabrera, Klaus Kratochwill, Sotirios G. Zarogiannis and Claus Peter Schmitt |
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| E-Jahr: | 2020 |
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| Jahr: | 13 August 2020 |
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| Fussnoten: | Gesehen am 12.11.2020 |
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| Titel Quelle: | Enthalten in: Biomolecules |
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| Ort Quelle: | Basel : MDPI, 2011 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 10(2020,8) Artikel-Nummer 1178, 17 Seiten |
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| ISSN Quelle: | 2218-273X |
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| Abstract: | Understanding and targeting the molecular basis of peritoneal solute and protein transport is essential to improve peritoneal dialysis (PD) efficacy and patient outcome. Supplementation of PD fluids (PDF) with alanyl-glutamine (AlaGln) increased small solute transport and reduced peritoneal protein loss in a recent clinical trial. Transepithelial resistance and 10 kDa and 70 kDa dextran transport were measured in primary human endothelial cells (HUVEC) exposed to conventional acidic, glucose degradation products (GDP) containing PDF (CPDF) and to low GDP containing PDF (LPDF) with and without AlaGln. Zonula occludens-1 (ZO-1) and claudin-5 were quantified by Western blot and immunofluorescence and in mice exposed to saline and CPDF for 7 weeks by digital imaging analyses. Spatial clustering of ZO-1 molecules was assessed by single molecule localization microscopy. AlaGln increased transepithelial resistance, and in CPDF exposed HUVEC decreased dextran transport rates and preserved claudin-5 and ZO-1 abundance. Endothelial clustering of membrane bound ZO-1 was higher in CPDF supplemented with AlaGln. In mice, arteriolar endothelial claudin-5 was reduced in CPDF, but restored with AlaGln, while mesothelial claudin-5 abundance was unchanged. AlaGln supplementation seals the peritoneal endothelial barrier, and when supplemented to conventional PD fluid increases claudin-5 and ZO-1 abundance and clustering of ZO-1 in the endothelial cell membrane. |
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| DOI: | doi:10.3390/biom10081178 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/biom10081178 |
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| | Volltext: https://www.mdpi.com/2218-273X/10/8/1178 |
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| | DOI: https://doi.org/10.3390/biom10081178 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | alanyl-glutamine |
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| | paracellular transport |
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| | peritoneal dialysis |
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| | tight junctions |
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| K10plus-PPN: | 1738550451 |
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| Verknüpfungen: | → Zeitschrift |
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Alanyl-glutamine restores tight junction organization after disruption by a conventional peritoneal dialysis fluid / Bartosova, Maria [VerfasserIn]; 13 August 2020 (Online-Ressource)
