| |  Online-Ressource |
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| Verfasst von: | Braet, Filip [VerfasserIn]  |
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| | Cheng, Delfine [VerfasserIn] |
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| | Huynh, Minh [VerfasserIn] |
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| | Henriquez, Jeffrey [VerfasserIn] |
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| | Shami, Gerry [VerfasserIn] |
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| | Lampe, Marko [VerfasserIn] |
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| Titel: | Combining wide-field super-resolution microscopy and electron tomography |
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| Titelzusatz: | rendering nanoscopic correlative arrays on subcellular architecture |
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| Verf.angabe: | Filip Braet, Delfine Cheng, Minh Huynh, Jeffrey Henriquez, Gerry Shami, Marko Lampe |
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| E-Jahr: | 2014 |
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| Jahr: | 3 October 2014 |
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| Umfang: | 21 S. |
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| Fussnoten: | Gesehen am 12.11.2020 |
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| Titel Quelle: | Enthalten in: Correlative light and electron microscopy II |
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| Ausgabe Quelle: | First edition |
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| Ort Quelle: | San Diego : Elsevier/Academic Press, 2014 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | (2014), Seite 129-149 |
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| Abstract: | In this chapter, the authors outline in full detail, an uncomplicated approach that enables the combination of wide-field fluorescence super-resolution microscopy with electron tomography, thereby providing an approach that affords the best possible confidence in the structures investigated. The methodical steps to obtain these high-throughput correlative nanoscopic arrays will be visually explored and outlined in detail. The authors will demonstrate the feasibility of the method on cultured Caco-2 colorectal cancer cells that are labeled for filamentous actin. The presented images, morphometric data, and generated models illustrate the strengths of our correlative approach for future advanced structural-biology-oriented questions. Correlative nanoscopy applications can be readily found in which there is a need to reveal biomolecular information at unprecedented resolution on subcellular behavior in various biological and pathobiological processes. |
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| DOI: | doi:10.1016/B978-0-12-801075-4.00007-0 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag ; Resolving-System: http://www.sciencedirect.com/science/article/pii/B9780128010754000070 |
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| | Volltext: https://doi.org/10.1016/B978-0-12-801075-4.00007-0 |
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| | DOI: https://doi.org/10.1016/B978-0-12-801075-4.00007-0 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Actin |
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| | Colon cancer |
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| | Correlating single molecules |
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| | Correlative morphomics |
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| | Correlative nanoscopy |
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| | Correlative pointillism |
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| | Cytoskeleton |
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| | Fine structure immunocytochemistry |
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| | Fluorescent probes |
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| | Ground state depletion |
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| | Nanoscopy |
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| | Simulation |
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| | Three-dimensional modeling |
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| K10plus-PPN: | 1738639096 |
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| Verknüpfungen: | → Sammelwerk |
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Combining wide-field super-resolution microscopy and electron tomography / Braet, Filip [VerfasserIn]; 3 October 2014 (Online-Ressource)
