| |  Online-Ressource |
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| Verfasst von: | Stelmach, Patrick [VerfasserIn]  |
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| | Pütz, Michael [VerfasserIn] |
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| | Pollmann, Robert [VerfasserIn] |
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| | Happel, Michael [VerfasserIn] |
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| | Stei, Susanne [VerfasserIn] |
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| | Schlegel, Kerstin [VerfasserIn] |
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| | Seipelt, Maria [VerfasserIn] |
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| | Eienbröker, Christian [VerfasserIn] |
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| | Eming, Rüdiger [VerfasserIn] |
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| | Mandic, Robert [VerfasserIn] |
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| | Huber, Magdalena [VerfasserIn] |
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| | Tackenberg, Björn [VerfasserIn] |
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| Titel: | Alternative splicing of the TNFSF13B (BAFF) pre-mRNA and expression of the BAFFX1 isoform in human immune cells |
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| Verf.angabe: | Patrick Stelmach, Michael Pütz, Robert Pollmann, Michael Happel, Susanne Stei, Kerstin Schlegel, Maria Seipelt, Christian Eienbröker, Rüdiger Eming, Robert Mandic, Magdalena Huber, Björn Tackenberg |
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| E-Jahr: | 2020 |
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| Jahr: | 04 August 2020 |
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| Umfang: | 8 S. |
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| Fussnoten: | Gesehen am 17.11.2020 |
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| Titel Quelle: | Enthalten in: Gene |
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| Ort Quelle: | Amsterdam : Elsevier, 1976 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 760(2020) Artikel-Nummer 145021, 8 Seiten |
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| ISSN Quelle: | 1879-0038 |
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| Abstract: | Human B cell activating factor (TNFSF13B, BAFF) is a tumor necrosis factor superfamily member. Binding its unique receptor (TNFRSF13C, BAFF-R) mediates gene expression and cell survival in B cells via activation of NFκB pathway. Furthermore, there is data indicating a role in T cell function. A functionally inhibitory isoform (ΔBAFF) resulting from the deletion of exon 3 in the TNFSF13B pre-RNA has already been reported. However, data on the complexity of post-transcriptional regulation is scarce. Here, we report molecular cloning of nine TNFSF13B transcript variants resulting from alternative splicing of the TNFSF13B pre-mRNA including BAFFX1. This variant is characterized by a partial retention of intron 3 of the TNFSF13B gene causing the appearance of a premature stop codon. We demonstrate the expression of the corresponding BAFFX1 protein in Jurkat T cells, in ex vivo human immune cells and in human tonsillar tissue. Thereby we contribute to the understanding of TNFSF13B gene regulation and reveal that BAFF is regulated through a post-transcriptional mechanism to a greater extent than reported to date. |
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| DOI: | doi:10.1016/j.gene.2020.145021 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.gene.2020.145021 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0378111920306909 |
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| | DOI: https://doi.org/10.1016/j.gene.2020.145021 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | B cell activating factor |
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| | BAFF |
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| | BAFFX1 |
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| | Post-transcriptional modification |
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| | TNFSF13B |
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| K10plus-PPN: | 173895367X |
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| Verknüpfungen: | → Zeitschrift |
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Alternative splicing of the TNFSF13B (BAFF) pre-mRNA and expression of the BAFFX1 isoform in human immune cells / Stelmach, Patrick [VerfasserIn]; 04 August 2020 (Online-Ressource)
