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Verfasst von:Fitterer, Raphaela [VerfasserIn]   i
 Lajqi, Trim [VerfasserIn]   i
 Kranig, Simon A. [VerfasserIn]   i
 Braun, Maylis [VerfasserIn]   i
 Theissig, Nicole [VerfasserIn]   i
 Kuß, Navina [VerfasserIn]   i
 Pöschl, Johannes [VerfasserIn]   i
 Frommhold, David [VerfasserIn]   i
 Hudalla, Hannes [VerfasserIn]   i
Titel:L-arginine modulates neonatal leukocyte recruitment in a gestational age-dependent manner
Verf.angabe:Raphaela Fitterer, Trim Lajqi, Simon Alexander Kranig, Maylis Braun, Nicole Theissig, Navina Kuss, Johannes Pöschl, David Frommhold and Hannes Hudalla
E-Jahr:2020
Jahr:27 August 2020
Umfang:13 S.
Fussnoten:Gesehen am 19.11.2020
Titel Quelle:Enthalten in: Journal of Clinical Medicine
Ort Quelle:Basel : MDPI, 2012
Jahr Quelle:2020
Band/Heft Quelle:9(2020,9) Artikel-Nummer 2772, 13 Seiten
ISSN Quelle:2077-0383
Abstract:(1) Background: L-arginine is a complex modulator of immune functions, and its levels are known to decrease under septic conditions. L-arginine may suppress leukocyte recruitment in vivo; however, little is known about the gestational age-specific effects of L-arginine on leukocyte recruitment in preterm infants. We now asked whether L-arginine alters leukocyte recruitment in preterm and term neonates. (2) Methods: Leukocytes were isolated from preterm (28 + 0 to 32 + 6 weeks of gestation) and term (>37 weeks of gestation) newborns as well as from healthy adults. After incubation with 10 µg/mL L-arginine, we assessed leukocyte rolling and adhesion in dynamic microflow chamber experiments and leukocyte transmigration in fluorescence assays. In addition, we measured the expression of inducible nitric oxide synthase (iNOS) and Arginase 1 (Arg-1) in neutrophils by flow cytometry. (3) Results: Leukocyte rolling, adhesion, and transmigration increased with gestational age. Leukocyte rolling, adhesion, and transmigration were decreased by L-arginine in term-born infants and adults. Preterm leukocytes showed no change in recruitment upon L-arginine exposure. Leukocyte adhesion after L-arginine exposure reached similar levels among all groups. In line, the expression of iNOS and Arg-1 was similar in all three age groups. (4) Conclusion: L-arginine dampens the ex vivo recruitment capacity of leukocytes from term-born infants, whereas no effect was seen in premature infants. As levels of iNOS and Arg-1 in neutrophils remain ontogenetically unchanged, the anti-inflammatory effect of L-arginine on the leukocyte recruitment cascade needs further investigation. These results add to the controversial debate of L-arginine supplementation in premature infants in sepsis.
DOI:doi:10.3390/jcm9092772
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/jcm9092772
 Volltext: https://www.mdpi.com/2077-0383/9/9/2772
 DOI: https://doi.org/10.3390/jcm9092772
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:innate immunity
 L-arginine
 leukocyte recruitment
 neutrophils
 newborn
K10plus-PPN:1739115414
Verknüpfungen:→ Zeitschrift

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