| Online-Ressource |
Verfasst von: | Fitterer, Raphaela [VerfasserIn]  |
| Lajqi, Trim [VerfasserIn]  |
| Kranig, Simon A. [VerfasserIn]  |
| Braun, Maylis [VerfasserIn]  |
| Theissig, Nicole [VerfasserIn]  |
| Kuß, Navina [VerfasserIn]  |
| Pöschl, Johannes [VerfasserIn]  |
| Frommhold, David [VerfasserIn]  |
| Hudalla, Hannes [VerfasserIn]  |
Titel: | L-arginine modulates neonatal leukocyte recruitment in a gestational age-dependent manner |
Verf.angabe: | Raphaela Fitterer, Trim Lajqi, Simon Alexander Kranig, Maylis Braun, Nicole Theissig, Navina Kuss, Johannes Pöschl, David Frommhold and Hannes Hudalla |
E-Jahr: | 2020 |
Jahr: | 27 August 2020 |
Umfang: | 13 S. |
Fussnoten: | Gesehen am 19.11.2020 |
Titel Quelle: | Enthalten in: Journal of Clinical Medicine |
Ort Quelle: | Basel : MDPI, 2012 |
Jahr Quelle: | 2020 |
Band/Heft Quelle: | 9(2020,9) Artikel-Nummer 2772, 13 Seiten |
ISSN Quelle: | 2077-0383 |
Abstract: | (1) Background: L-arginine is a complex modulator of immune functions, and its levels are known to decrease under septic conditions. L-arginine may suppress leukocyte recruitment in vivo; however, little is known about the gestational age-specific effects of L-arginine on leukocyte recruitment in preterm infants. We now asked whether L-arginine alters leukocyte recruitment in preterm and term neonates. (2) Methods: Leukocytes were isolated from preterm (28 + 0 to 32 + 6 weeks of gestation) and term (>37 weeks of gestation) newborns as well as from healthy adults. After incubation with 10 µg/mL L-arginine, we assessed leukocyte rolling and adhesion in dynamic microflow chamber experiments and leukocyte transmigration in fluorescence assays. In addition, we measured the expression of inducible nitric oxide synthase (iNOS) and Arginase 1 (Arg-1) in neutrophils by flow cytometry. (3) Results: Leukocyte rolling, adhesion, and transmigration increased with gestational age. Leukocyte rolling, adhesion, and transmigration were decreased by L-arginine in term-born infants and adults. Preterm leukocytes showed no change in recruitment upon L-arginine exposure. Leukocyte adhesion after L-arginine exposure reached similar levels among all groups. In line, the expression of iNOS and Arg-1 was similar in all three age groups. (4) Conclusion: L-arginine dampens the ex vivo recruitment capacity of leukocytes from term-born infants, whereas no effect was seen in premature infants. As levels of iNOS and Arg-1 in neutrophils remain ontogenetically unchanged, the anti-inflammatory effect of L-arginine on the leukocyte recruitment cascade needs further investigation. These results add to the controversial debate of L-arginine supplementation in premature infants in sepsis. |
DOI: | doi:10.3390/jcm9092772 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/jcm9092772 |
| Volltext: https://www.mdpi.com/2077-0383/9/9/2772 |
| DOI: https://doi.org/10.3390/jcm9092772 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | innate immunity |
| L-arginine |
| leukocyte recruitment |
| neutrophils |
| newborn |
K10plus-PPN: | 1739115414 |
Verknüpfungen: | → Zeitschrift |
L-arginine modulates neonatal leukocyte recruitment in a gestational age-dependent manner / Fitterer, Raphaela [VerfasserIn]; 27 August 2020 (Online-Ressource)