L-arginine modulates neonatal leukocyte recruitment in a gestational age-dependent manner

• Verfasst von: Fitterer, Raphaela [VerfasserIn]
• Verfasst von: Lajqi, Trim [VerfasserIn]
• Verfasst von: Kranig, Simon A. [VerfasserIn]
• Verfasst von: Braun, Maylis [VerfasserIn]
• Verfasst von: Theissig, Nicole [VerfasserIn]
• Verfasst von: Kuß, Navina [VerfasserIn]
• Verfasst von: Pöschl, Johannes [VerfasserIn]
• Verfasst von: Frommhold, David [VerfasserIn]
• Verfasst von: Hudalla, Hannes [VerfasserIn]
• Titel: L-arginine modulates neonatal leukocyte recruitment in a gestational age-dependent manner
• Verf.angabe: Raphaela Fitterer, Trim Lajqi, Simon Alexander Kranig, Maylis Braun, Nicole Theissig, Navina Kuss, Johannes Pöschl, David Frommhold and Hannes Hudalla
• E-Jahr: 2020
• Jahr: 27 August 2020
• Umfang: 13 S.
• Fussnoten: Gesehen am 19.11.2020
• Titel Quelle: Enthalten in: Journal of Clinical Medicine
• Ort Quelle: Basel : MDPI, 2012
• Jahr Quelle: 2020
• Band/Heft Quelle: 9(2020,9) Artikel-Nummer 2772, 13 Seiten
• ISSN Quelle: 2077-0383
• DOI: doi:10.3390/jcm9092772
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: innate immunity
• Sach-SW: L-arginine
• Sach-SW: leukocyte recruitment
• Sach-SW: neutrophils
• Sach-SW: newborn
• K10plus-PPN: 1739115414

Abstract

(1) Background: L-arginine is a complex modulator of immune functions, and its levels are known to decrease under septic conditions. L-arginine may suppress leukocyte recruitment in vivo; however, little is known about the gestational age-specific effects of L-arginine on leukocyte recruitment in preterm infants. We now asked whether L-arginine alters leukocyte recruitment in preterm and term neonates. (2) Methods: Leukocytes were isolated from preterm (28 + 0 to 32 + 6 weeks of gestation) and term (>37 weeks of gestation) newborns as well as from healthy adults. After incubation with 10 µg/mL L-arginine, we assessed leukocyte rolling and adhesion in dynamic microflow chamber experiments and leukocyte transmigration in fluorescence assays. In addition, we measured the expression of inducible nitric oxide synthase (iNOS) and Arginase 1 (Arg-1) in neutrophils by flow cytometry. (3) Results: Leukocyte rolling, adhesion, and transmigration increased with gestational age. Leukocyte rolling, adhesion, and transmigration were decreased by L-arginine in term-born infants and adults. Preterm leukocytes showed no change in recruitment upon L-arginine exposure. Leukocyte adhesion after L-arginine exposure reached similar levels among all groups. In line, the expression of iNOS and Arg-1 was similar in all three age groups. (4) Conclusion: L-arginine dampens the ex vivo recruitment capacity of leukocytes from term-born infants, whereas no effect was seen in premature infants. As levels of iNOS and Arg-1 in neutrophils remain ontogenetically unchanged, the anti-inflammatory effect of L-arginine on the leukocyte recruitment cascade needs further investigation. These results add to the controversial debate of L-arginine supplementation in premature infants in sepsis.