Computerized image analysis of iron-stained macrophages

• Verfasst von: Gente, Karolina [VerfasserIn]
• Verfasst von: Schäfer, Sebastian Markus [VerfasserIn]
• Verfasst von: Mall, Marcus A. [VerfasserIn]
• Verfasst von: Muckenthaler, Martina [VerfasserIn]
• Titel: Computerized image analysis of iron-stained macrophages
• Verf.angabe: Karolina Benesova, Sebastian M. Schaefer, Marcus A. Mall, Martina U. Muckenthaler
• E-Jahr: 2013
• Jahr: 17 April 2013
• Umfang: 5 S.
• Fussnoten: Gesehen am 23.11.2020
• Titel Quelle: Enthalten in: Annals of hematology
• Ort Quelle: Berlin : Springer, 1955
• Jahr Quelle: 2013
• Band/Heft Quelle: 92(2013), 9, Seite 1195-1199
• ISSN Quelle: 1432-0584
• DOI: doi:10.1007/s00277-013-1740-2
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1740378768

Abstract

Analysis of iron levels in single cells is critical to understand the consequences of impaired regulation of iron homeostasis. Here we establish a method to analyze intracellular iron deposits by computerized image analysis of Prussian blue-stained alveolar macrophages as a test system. We efficiently detected small differences in macrophage steady-state iron levels in Hfe−/− mice as well as inflammation-induced iron sequestration upon lipopolysaccharide instillation. In conclusion, computerized image analysis of single cells is a robust and reproducible tool suitable for iron measurements in small sample sets with limited cell yield.