Status: Bibliographieeintrag
Standort: ---
Exemplare:
---
| |  Online-Ressource |
|---|
| Verfasst von: | Nurjadi, Dennis [VerfasserIn]  |
|---|
| | Boutin, Sébastien [VerfasserIn] |
|---|
| | Schmidt, Katja [VerfasserIn] |
|---|
| | Ahmels, Melinda [VerfasserIn] |
|---|
| | Hasche, Daniel [VerfasserIn] |
|---|
| Titel: | Identification and elimination of the clinically relevant multi-resistant environmental bacteria Ralstonia insidiosa in primary cell culture |
|---|
| Verf.angabe: | Dennis Nurjadi, Sébastien Boutin, Katja Schmidt, Melinda Ahmels and Daniel Hasche |
|---|
| E-Jahr: | 2020 |
|---|
| Jahr: | 17 October 2020 |
|---|
| Umfang: | 12 S. |
|---|
| Fussnoten: | Gesehen am 23.10.2020 |
|---|
| Titel Quelle: | Enthalten in: Microorganisms |
|---|
| Ort Quelle: | Basel : MDPI, 2013 |
|---|
| Jahr Quelle: | 2020 |
|---|
| Band/Heft Quelle: | 8(2020,10) Artikel-Nummer 1599, 12 Seiten |
|---|
| ISSN Quelle: | 2076-2607 |
|---|
| Abstract: | In times of spreading multidrug-resistant bacteria, species identification and decontamination of cell cultures can be challenging. Here, we describe a mobile cell culture contaminant with “black dot”-like microscopic appearance in newly established irreplaceable hybridoma cell lines and its identification. Using 16S rRNA gene sequencing, species-specific PCRs, whole genome sequencing (WGS), and MALDI-TOF mass spectrometry, the contaminant was identified as the ubiquitous environmental and clinically relevant Gram-negative bacterium Ralstonia insidiosa (R. insidiosa), a strong biofilm producer. Further characterizations by transmission electron microscopy (TEM) and biochemical API test were not conclusive. Whole genome sequencing of our R. insidiosa isolate revealed numerous drug-resistance determinants. Genome-wide comparison to other Ralstonia species could not unambiguously designate our isolate to R. insidiosa (<95% average nucleotide identity) suggesting a potential novel species or subspecies, closely related to R. insidiosa and R. pickettii. After determining the antibiotic susceptibility profile, the hybridoma cell culture was successfully decontaminated with ciprofloxacin without affecting antibody production. |
|---|
| DOI: | doi:10.3390/microorganisms8101599 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/microorganisms8101599 |
|---|
| | Volltext: https://www.mdpi.com/2076-2607/8/10/1599 |
|---|
| | DOI: https://doi.org/10.3390/microorganisms8101599 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | <i>Ralstonia insidiosa</i> |
|---|
| | bacterial contamination |
|---|
| | multidrug resistance |
|---|
| | primary cell culture |
|---|
| K10plus-PPN: | 1740381637 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Identification and elimination of the clinically relevant multi-resistant environmental bacteria Ralstonia insidiosa in primary cell culture / Nurjadi, Dennis [VerfasserIn]; 17 October 2020 (Online-Ressource)
68664243
