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| Verfasst von: | Jarius, Sven [VerfasserIn]  |
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| | Scharf, Madeleine [VerfasserIn] |
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| | Begemann, Nora [VerfasserIn] |
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| | Stöcker, Winfried [VerfasserIn] |
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| | Probst, Christian [VerfasserIn] |
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| | Serysheva, Irina I. [VerfasserIn] |
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| | Nagel, Sigrun [VerfasserIn] |
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| | Graus, Francesc [VerfasserIn] |
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| | Psimaras, Dimitri [VerfasserIn] |
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| | Wildemann, Brigitte [VerfasserIn] |
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| | Komorowski, Lars [VerfasserIn] |
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| Titel: | Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia |
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| Verf.angabe: | Sven Jarius, Madeleine Scharf, Nora Begemann, Winfried Stöcker, Christian Probst, Irina I. Serysheva, Sigrun Nagel, Francesc Graus, Dimitri Psimaras, Brigitte Wildemann and Lars Komorowski |
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| E-Jahr: | 2014 |
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| Jahr: | 11 December 2014 |
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| Umfang: | 12 S. |
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| Fussnoten: | Gesehen am 24.11.2020 |
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| Titel Quelle: | Enthalten in: Journal of neuroinflammation |
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| Ort Quelle: | London : BioMed Central, 2004 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | 11(2014) Artikel-Nummer 206, 12 Seiten |
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| ISSN Quelle: | 1742-2094 |
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| Abstract: | We report on a serum autoantibody associated with cerebellar ataxia. Immunohistochemical studies of sera from four patients referred for autoantibody testing revealed binding of high-titer (up to 1:5,000) IgG antibodies, mainly IgG1, to the molecular layer, Purkinje cell layer, and white matter on mouse, rat, porcine, and monkey cerebellum sections. The antibody bound to PC somata, dendrites, and axons, resulting in a binding pattern similar to that reported for anti-Ca/anti-ARHGAP26, but did not react with recombinant ARHGAP26. Extensive control studies were performed to rule out a broad panel of previously described paraneoplastic and non-paraneoplastic anti-neural autoantibodies. The characteristic binding pattern as well as double staining experiments suggested inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) as the target antigen. Verification of the antigen included specific neutralization of the tissue reaction following preadsorption with ITPR1 (but not ARHGAP26) and a dot-blot assay with purified ITPR1 protein. By contrast, anti-ARHGAP26-positive sera did not bind to ITPR1. In a parallel approach, a combination of histoimmunoprecipitation and mass spectrometry also identified ITPR1 as the target antigen. Finally, a recombinant cell-based immunofluorescence assay using HEK293 cells expressing ITPR1 and ARHGAP26, respectively, confirmed the identification of ITPR1. Mutations of ITPR1 have previously been implicated in spinocerebellar ataxia with and without cognitive decline. Our findings suggest a role of autoimmunity against ITPR1 in the pathogenesis of autoimmune cerebellitis and extend the panel of diagnostic markers for this disease. |
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| DOI: | doi:10.1186/s12974-014-0206-3 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1186/s12974-014-0206-3 |
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| | DOI: https://doi.org/10.1186/s12974-014-0206-3 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Adult |
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| | Animals |
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| | Autoantibodies |
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| | Cerebellar Ataxia |
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| | Female |
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| | HEK293 Cells |
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| | Humans |
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| | Inositol 1,4,5-Trisphosphate Receptors |
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| | Macaca mulatta |
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| | Mice |
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| | Rats |
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| | Swine |
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| K10plus-PPN: | 1740864360 |
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| Verknüpfungen: | → Zeitschrift |
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Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia / Jarius, Sven [VerfasserIn]; 11 December 2014 (Online-Ressource)
