| |  Online-Ressource |
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| Verfasst von: | Aidaros, Anas Al [VerfasserIn]  |
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| | Sharma, Charu [VerfasserIn] |
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| | Langhans, Claus-Dieter [VerfasserIn] |
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| | Okun, Jürgen G. [VerfasserIn] |
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| | Hoffmann, Georg Friedrich [VerfasserIn] |
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| | Dasouki, Majed [VerfasserIn] |
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| | Chakraborty, Pranesh [VerfasserIn] |
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| | Aljasmi, Fatma [VerfasserIn] |
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| | Al-Dirbashi, Osama Y. [VerfasserIn] |
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| Titel: | Targeted metabolomic profiling of total fatty acids in human plasma by liquid chromatography-tandem mass spectrometry |
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| Verf.angabe: | Anas Al Aidaros, Charu Sharma, Claus-Dieter Langhans, Jürgen G. Okun, Georg F. Hoffmann, Majed Dasouki, Pranesh Chakraborty, Fatma Aljasmi and Osama Y. Al-Dirbashi |
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| E-Jahr: | 2020 |
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| Jahr: | 9 October 2020 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 25.11.2020 |
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| Titel Quelle: | Enthalten in: Metabolites |
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| Ort Quelle: | Basel : MDPI, 2011 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 10(2020,10) Artikel-Nummer 400, 15 Seiten |
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| ISSN Quelle: | 2218-1989 |
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| Abstract: | This article reports a targeted metabolomic method for total plasma fatty acids (FAs) of clinical or nutritional relevance. Thirty-six saturated, unsaturated, or branched-chain FAs with a chain length of C8-C28 were quantified using reversed-phase liquid chromatography-tandem mass spectrometry. FAs in plasma (10 μL) were acid-hydrolyzed, extracted, and derivatized with DAABD-AE (4-[2-(N,N-Dimethylamino)ethylaminosulfonyl]-7-(2-aminoethylamino)-2,1,3-benzoxadiazole) at 60 °C for 1 h. Derivatization resulted in a staggering nine orders of magnitude higher sensitivity compared to underivatized analytes. FAs were measured by multiple-reaction monitoring using stable isotope internal standards. With physiological and pathological analyte levels in mind, linearity was established using spiked plasma. Intra-day (n = 15) and inter-day (n = 20) imprecisions expressed as variation coefficient were ≤10.2% with recovery ranging between 94.5–106.4%. Limits of detection and limit of quantitation ranged between 4.2–14.0 and 15.1–51.3 pmol per injection, respectively. Age-stratified reference intervals were established in four categories: <1 month, 1–12 month, 1–18 year, and >18 year. This method was assessed using samples from patients with disorders affecting FAs metabolism. For the first time, C28:0 and C28:0/C22:0 ratio were evaluated as novel disease biomarkers. This method can potentially be utilized in diagnosing patients with inborn errors of metabolism, chronic disease risk estimation, or nutritional applications. |
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| DOI: | doi:10.3390/metabo10100400 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/metabo10100400 |
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| | Volltext: https://www.mdpi.com/2218-1989/10/10/400 |
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| | DOI: https://doi.org/10.3390/metabo10100400 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | derivatization |
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| | inborn errors of metabolism |
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| | liquid chromatography-tandem mass spectrometry |
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| | plasma fatty acids |
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| | targeted metabolomics |
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| K10plus-PPN: | 1741049393 |
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| Verknüpfungen: | → Zeitschrift |
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Targeted metabolomic profiling of total fatty acids in human plasma by liquid chromatography-tandem mass spectrometry / Aidaros, Anas Al [VerfasserIn]; 9 October 2020 (Online-Ressource)
