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Status: Bibliographieeintrag

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Verfasst von:Kolodziej, Malgorzata A. [VerfasserIn]   i
 Al Barim, B. [VerfasserIn]   i
 Nagl, J. [VerfasserIn]   i
 Weigand, Markus A. [VerfasserIn]   i
 Uhl, E. [VerfasserIn]   i
 Uhle, Florian [VerfasserIn]   i
 Di Fazio, P. [VerfasserIn]   i
 Schwarm, F. P. [VerfasserIn]   i
 Stein, M. [VerfasserIn]   i
Titel:Sphingosine-1-phosphate analogue FTY720 exhibits a potent anti-proliferative effect on glioblastoma cells
Verf.angabe:M.A. Kolodziej, B. Al Barim, J. Nagl, M.A. Weigand, E. Uhl, F. Uhle, P. Di Fazio, F.P. Schwarm, M. Stein
E-Jahr:2020
Jahr:August 25, 2020
Umfang:8 S.
Fussnoten:Gesehen am 27.11.2020
Titel Quelle:Enthalten in: International journal of oncology
Ort Quelle:Athens : Spandidos Publ., 2001
Jahr Quelle:2020
Band/Heft Quelle:57(2020), 4, Seite 1039-1046
ISSN Quelle:1791-2423
Abstract:Sphingosine-1-phosphate (S1P) plays a key role in cell survival, growth, migration, and in angiogenesis. In glioma, it triggers the activity of the S1P-receptor 1 and of the sphingosine kinase 1; thus influencing the survival rate of patients. The aim of the present study was to investigate the anti-proliferative effect of the S1P analogue FTY720 (fingolimod) in glioblastoma (GBM) cells. A172, G28, and U87 cells were incubated with micromolar concentrations of FTY720 or temozolomide (TMZ) for 24 to 72 h. Proliferation and half maximal inhibitory concentration (IC50) were determined by using the xCELLigence system. FACS analysis was performed to check the cell cycle distribution of the cells after a 72-h incubation with FTY720. This was then compared to TMZ-incubated and to untreated cells. Gene expression was detected by RT-qPCR in A172, G28, U87 and three primary GBM-derived cell lines. FTY720 was able to reduce the number of viable cells. The IC50 value was 4.6 mu M in A172 cells, 17.3 mu M in G28 cells, and 25.2 mu M in U87 cells. FTY720 caused a significant arrest of the cell cycle in all cells and stabilized or over-expressed the level of AKT1, MAPK1, PKCE, RAC1, and ROCK1 transcripts. The TP53 transcript level remained stable or was downregulated after treatment with FTY720. FTY720 may be a promising target drug for the treatment of GBM, as it has a strong anti-proliferative effect on GBM cells.
DOI:doi:10.3892/ijo.2020.5114
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3892/ijo.2020.5114
 DOI: https://doi.org/10.3892/ijo.2020.5114
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:1-phosphate
 activation
 apoptosis
 astrocytes
 cell death
 cell viability
 death
 fingolimod
 fty720
 glioblastoma multiforme
 growth
 multiforme
 receptors
 sphingosine kinase isoforms
 temozolomide
 tumor-derived cells
K10plus-PPN:1741331250
Verknüpfungen:→ Zeitschrift

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