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Status: Bibliographieeintrag

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Verfasst von:Ullate-Agote, Asier [VerfasserIn]   i
 Burgelin, Ingrid [VerfasserIn]   i
 Debry, Adrien [VerfasserIn]   i
 Langrez, Carine [VerfasserIn]   i
 Montange, Florent [VerfasserIn]   i
 Peraldi, Rodrigue [VerfasserIn]   i
 Daraspe, Jean [VerfasserIn]   i
 Kaessmann, Henrik [VerfasserIn]   i
 Milinkovitch, Michel C. [VerfasserIn]   i
 Tzika, Athanasia C. [VerfasserIn]   i
Titel:Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles
Verf.angabe:Asier Ullate-Agote, Ingrid Burgelin, Adrien Debry, Carine Langrez, Florent Montange, Rodrigue Peraldi, Jean Daraspe, Henrik Kaessmann, Michel C. Milinkovitch, and Athanasia C. Tzika
E-Jahr:2020
Jahr:October 5, 2020
Umfang:11 S.
Fussnoten:Gesehen am 01.12.2020
Titel Quelle:Enthalten in: National Academy of Sciences (Washington, DC)Proceedings of the National Academy of Sciences of the United States of America
Ort Quelle:Washington, DC : National Acad. of Sciences, 1915
Jahr Quelle:2020
Band/Heft Quelle:117(2020), 42, Seite 26307-26317
ISSN Quelle:1091-6490
Abstract:Reptiles exhibit a spectacular diversity of skin colors and patterns brought about by the interactions among three chromatophore types: black melanophores with melanin-packed melanosomes, red and yellow xanthophores with pteridine- and/or carotenoid-containing vesicles, and iridophores filled with light-reflecting platelets generating structural colors. Whereas the melanosome, the only color-producing endosome in mammals and birds, has been documented as a lysosome-related organelle, the maturation paths of xanthosomes and iridosomes are unknown. Here, we first use 10x Genomics linked-reads and optical mapping to assemble and annotate a nearly chromosome-quality genome of the corn snake Pantherophis guttatus. The assembly is 1.71 Gb long, with an N50 of 16.8 Mb and L50 of 24. Second, we perform mapping-by-sequencing analyses and identify a 3.9-Mb genomic interval where the lavender variant resides. The lavender color morph in corn snakes is characterized by gray, rather than red, blotches on a pink, instead of orange, background. Third, our sequencing analyses reveal a single nucleotide polymorphism introducing a premature stop codon in the lysosomal trafficking regulator gene (LYST) that shortens the corresponding protein by 603 amino acids and removes evolutionary-conserved domains. Fourth, we use light and transmission electron microscopy comparative analyses of wild type versus lavender corn snakes and show that the color-producing endosomes of all chromatophores are substantially affected in the LYST mutant. Our work provides evidence characterizing xanthosomes in xanthophores and iridosomes in iridophores as lysosome-related organelles.
DOI:doi:10.1073/pnas.2003724117
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1073/pnas.2003724117
 Volltext: https://www.pnas.org/content/117/42/26307
 DOI: https://doi.org/10.1073/pnas.2003724117
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:chromatophores
 corn snake
 lysosome-related organelles
 LYST
 pigmentation
K10plus-PPN:174154808X
Verknüpfungen:→ Zeitschrift

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