| Online-Ressource |
Verfasst von: | Ullate-Agote, Asier [VerfasserIn]  |
| Burgelin, Ingrid [VerfasserIn]  |
| Debry, Adrien [VerfasserIn]  |
| Langrez, Carine [VerfasserIn]  |
| Montange, Florent [VerfasserIn]  |
| Peraldi, Rodrigue [VerfasserIn]  |
| Daraspe, Jean [VerfasserIn]  |
| Kaessmann, Henrik [VerfasserIn]  |
| Milinkovitch, Michel C. [VerfasserIn]  |
| Tzika, Athanasia C. [VerfasserIn]  |
Titel: | Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles |
Verf.angabe: | Asier Ullate-Agote, Ingrid Burgelin, Adrien Debry, Carine Langrez, Florent Montange, Rodrigue Peraldi, Jean Daraspe, Henrik Kaessmann, Michel C. Milinkovitch, and Athanasia C. Tzika |
E-Jahr: | 2020 |
Jahr: | October 5, 2020 |
Umfang: | 11 S. |
Fussnoten: | Gesehen am 01.12.2020 |
Titel Quelle: | Enthalten in: National Academy of Sciences (Washington, DC)Proceedings of the National Academy of Sciences of the United States of America |
Ort Quelle: | Washington, DC : National Acad. of Sciences, 1915 |
Jahr Quelle: | 2020 |
Band/Heft Quelle: | 117(2020), 42, Seite 26307-26317 |
ISSN Quelle: | 1091-6490 |
Abstract: | Reptiles exhibit a spectacular diversity of skin colors and patterns brought about by the interactions among three chromatophore types: black melanophores with melanin-packed melanosomes, red and yellow xanthophores with pteridine- and/or carotenoid-containing vesicles, and iridophores filled with light-reflecting platelets generating structural colors. Whereas the melanosome, the only color-producing endosome in mammals and birds, has been documented as a lysosome-related organelle, the maturation paths of xanthosomes and iridosomes are unknown. Here, we first use 10x Genomics linked-reads and optical mapping to assemble and annotate a nearly chromosome-quality genome of the corn snake Pantherophis guttatus. The assembly is 1.71 Gb long, with an N50 of 16.8 Mb and L50 of 24. Second, we perform mapping-by-sequencing analyses and identify a 3.9-Mb genomic interval where the lavender variant resides. The lavender color morph in corn snakes is characterized by gray, rather than red, blotches on a pink, instead of orange, background. Third, our sequencing analyses reveal a single nucleotide polymorphism introducing a premature stop codon in the lysosomal trafficking regulator gene (LYST) that shortens the corresponding protein by 603 amino acids and removes evolutionary-conserved domains. Fourth, we use light and transmission electron microscopy comparative analyses of wild type versus lavender corn snakes and show that the color-producing endosomes of all chromatophores are substantially affected in the LYST mutant. Our work provides evidence characterizing xanthosomes in xanthophores and iridosomes in iridophores as lysosome-related organelles. |
DOI: | doi:10.1073/pnas.2003724117 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1073/pnas.2003724117 |
| Volltext: https://www.pnas.org/content/117/42/26307 |
| DOI: https://doi.org/10.1073/pnas.2003724117 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | chromatophores |
| corn snake |
| lysosome-related organelles |
| LYST |
| pigmentation |
K10plus-PPN: | 174154808X |
Verknüpfungen: | → Zeitschrift |
Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles / Ullate-Agote, Asier [VerfasserIn]; October 5, 2020 (Online-Ressource)