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Verfasst von:Port, Fillip [VerfasserIn]   i
 Starostecka, Maja [VerfasserIn]   i
 Boutros, Michael [VerfasserIn]   i
Titel:Multiplexed conditional genome editing with Cas12a in Drosophila
Verf.angabe:Fillip Port, Maja Starostecka, and Michael Boutros
E-Jahr:2020
Jahr:August 25, 2020
Umfang:10 S.
Fussnoten:Gesehen am 04.12.2020
Titel Quelle:Enthalten in: National Academy of Sciences (Washington, DC)Proceedings of the National Academy of Sciences of the United States of America
Ort Quelle:Washington, DC : National Acad. of Sciences, 1915
Jahr Quelle:2020
Band/Heft Quelle:117(2020), 37, Seite 22890-22899
ISSN Quelle:1091-6490
Abstract:CRISPR-Cas genome engineering has revolutionized biomedical research by enabling targeted genomemodificationwith unprecedented ease. In the popular model organism Drosophila melanogaster, gene editing has so far relied exclusively on the prototypical CRISPR nuclease Cas9. Additional CRISPR systems could expand the genomic target space, offer additional modes of regulation, and enable the independent manipulation of genes in different cells of the same animal. Here we describe a platform for efficient Cas12a gene editing in Drosophila. We show that Cas12a from Lachnospiraceae bacterium, but not Acid-aminococcus spec., can mediate robust gene editing in vivo. In combination with most CRISPR RNAs (crRNAs), LbCas12a activity is high at 29 degrees C, but low at 18 degrees C, enabling modulation of gene editing by temperature. LbCas12a can directly utilize compact crRNA arrays that are substantially easier to construct than Cas9 single-guide RNA arrays, facilitating multiplex genome engineering. Furthermore, we showthat conditional expression of LbCas12a is sufficient to mediate tightly controlled gene editing in a variety of tissues, allowing detailed analysis of gene function in a multicellular organism. We also test a variant of LbCas12a with a D156R point mutation and show that it has substantially higher activity and outperforms a state-of-the-art Cas9 system in identifying essential genes. Cas12a gene editing expands the genomeengineering toolbox in Drosophila and will be a powerful method for the functional annotation of the genome. This work also presents a fully genetically encoded Cas12a system in an animal, laying out principles for the development of similar systems in other genetically tractable organisms for multiplexed conditional genome engineering.
DOI:doi:10.1073/pnas.2004655117
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1073/pnas.2004655117
 Volltext: https://www.pnas.org/content/117/37/22890
 DOI: https://doi.org/10.1073/pnas.2004655117
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cas12a
 Cas9
 cpf1
 crispr
 Drosophila
 expression
 genome engineering
 germline
 platform
 rna-guided endonuclease
 specificities
 toolkit
K10plus-PPN:1742032389
Verknüpfungen:→ Zeitschrift

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