HIV-specific CD8+ T lymphocytes in blood of long-term HIV-infected hemophilia patients

• Verfasst von: Daniel, Volker [VerfasserIn]
• Verfasst von: Scherer, Sabine [VerfasserIn]
• Verfasst von: Sadeghi, Mahmoud [VerfasserIn]
• Verfasst von: Terness, Peter [VerfasserIn]
• Verfasst von: Huth-Kühne, Angela [VerfasserIn]
• Verfasst von: Opelz, Gerhard [VerfasserIn]
• Titel: HIV-specific CD8+ T lymphocytes in blood of long-term HIV-infected hemophilia patients
• Verf.angabe: Volker Daniel, Sabine Scherer, Mahmoud Sadeghi, Peter Terness, Angela Huth-Kühne, and Gerhard Opelz
• E-Jahr: 2013
• Jahr: 17 December 2013
• Umfang: 13 S.
• Fussnoten: Gesehen am 09.12.2020
• Titel Quelle: Enthalten in: BioResearch open access
• Ort Quelle: New Rochelle, NY : Liebert, 2012
• Jahr Quelle: 2013
• Band/Heft Quelle: 2(2013), 6, Seite 399-411
• ISSN Quelle: 2164-7860
• DOI: doi:10.1089/biores.2013.0034
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1742399266

Abstract

Hemophilia patients infected with human immunodeficiency virus (HIV) 30 years ago show increased proportions of activated CD8+DR+ blood lymphocytes. We hypothesized that this might indicate a cellular immune response directed against HIV and might be the reason for long-term clinical stability of these patients. CD8+ peripheral blood lymphocytes (PBL) reactive with six HIV and two cytomegalovirus (CMV) pentamers were determined in heparinized whole blood. Additional lymphocyte subsets as well as plasma cytokines and HIV-1 load were studied. Long-term HIV-infected hemophilia patients with (n=15) or without (n=33) currently detectable HIV-1 load in the plasma showed higher proportions of CD8+ lymphocytes reactive with HIV (p<0.001) and CMV pentamers (p=0.010) than healthy individuals. The cellular anti-HIV response tended to be stronger and more polyclonal in patients during periods of viral replication than in patients with retroviral quiescence (p=0.077). Anti-HIV CD8+ lymphocyte responses were strongest in patients with high counts of activated CD8+DR+ T (r=0.353; p=0.014) and low CD19+ B lymphocyte counts (r=−0.472; p=0.001). Patients with or without HIV-1 viral load showed normal Th1 and Th2 plasma cytokine levels and high plasma interleukin-6 (versus healthy controls, p=0.001) and tumor necrosis factor-α (p=0.020). Hemophilia patients who have been living with HIV for more than 30 years showed a polyclonal CD8+ T-cell response against HIV and CMV. This cellular antiviral immune response was strongest during periods of HIV-1 replication and remained detectable during periods of HIV-1 quiescence. We hypothesize that the consistent cellular anti-HIV-1 response in combination with highly active antiretroviral therapy ensures stability and survival of these chronically HIV-1-infected hemophilia patients.