T cell activation is determined by the number of presented antigens

• Verfasst von: Deeg, Janosch A. [VerfasserIn]
• Verfasst von: Axmann, Markus [VerfasserIn]
• Verfasst von: Matić, Jovana [VerfasserIn]
• Verfasst von: Liapis, Anastasia [VerfasserIn]
• Verfasst von: Depoil, David [VerfasserIn]
• Verfasst von: Afrose, Jehan [VerfasserIn]
• Verfasst von: Curado, Silvia [VerfasserIn]
• Verfasst von: Dustin, Michael L. [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: T cell activation is determined by the number of presented antigens
• Verf.angabe: Janosch Deeg, Markus Axmann, Jovana Matic, Anastasia Liapis, David Depoil, Jehan Afrose, Silvia Curado, Michael L. Dustin, and Joachim P. Spatz
• E-Jahr: 2013
• Jahr: October 11, 2013
• Umfang: 8 S.
• Fussnoten: Gesehen am 10.12.2020
• Titel Quelle: Enthalten in: Nano letters
• Ort Quelle: Washington, DC : ACS Publ., 2001
• Jahr Quelle: 2013
• Band/Heft Quelle: 13(2013), 11, Seite 5619-5626
• ISSN Quelle: 1530-6992
• DOI: doi:10.1021/nl403266t
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1742490093

Abstract

Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm2. We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.