CDK1 couples proliferation with protein synthesis

• Verfasst von: Hörth, Katharina [VerfasserIn]
• Verfasst von: Schott, Johanna [VerfasserIn]
• Verfasst von: Lindner, Doris [VerfasserIn]
• Verfasst von: Kruse Hollensen, Anne [VerfasserIn]
• Verfasst von: Kroun Damgaard, Christian [VerfasserIn]
• Verfasst von: Mongis, Cyril [VerfasserIn]
• Verfasst von: Knop, Michael [VerfasserIn]
• Verfasst von: Palm, Wilhelm [VerfasserIn]
• Verfasst von: Ruggieri, Alessia [VerfasserIn]
• Verfasst von: Stoecklin, Georg [VerfasserIn]
• Titel: CDK1 couples proliferation with protein synthesis
• Verf.angabe: Katharina Haneke, Johanna Schott, Doris Lindner, Anne Kruse Hollensen, Christian Kroun Damgaard, Cyril Mongis, Michael Knop, Wilhelm Palm, Alessia Ruggieri, and Georg Stoecklin
• E-Jahr: 2020
• Jahr: February 10 2020
• Umfang: 23 S.
• Fussnoten: Gesehen am 17.12.2020
• Titel Quelle: Enthalten in: The journal of cell biology
• Ort Quelle: New York, NY : Rockefeller Univ. Press, 1962
• Jahr Quelle: 2020
• Band/Heft Quelle: 219(2020), 3, Artikel-ID e201906147, Seite 1-23
• ISSN Quelle: 1540-8140
• DOI: doi:10.1083/jcb.201906147
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Forschungsdaten: Hörth, Katharina: CDK1 couples proliferation with protein synthesis [repository tables R1-R4]
• K10plus-PPN: 1743132980

Abstract

Cell proliferation exerts a high demand on protein synthesis, yet the mechanisms coupling the two processes are not fully understood. A kinase and phosphatase screen for activators of translation, based on the formation of stress granules in human cells, revealed cell cycle-associated kinases as major candidates. CDK1 was identified as a positive regulator of global translation, and cell synchronization experiments showed that this is an extramitotic function of CDK1. Different pathways including eIF2α, 4EBP, and S6K1 signaling contribute to controlling global translation downstream of CDK1. Moreover, Ribo-Seq analysis uncovered that CDK1 exerts a particularly strong effect on the translation of 5′TOP mRNAs, which includes mRNAs encoding ribosomal proteins and several translation factors. This effect requires the 5′TOP mRNA-binding protein LARP1, concurrent to our finding that LARP1 phosphorylation is strongly dependent on CDK1. Thus, CDK1 provides a direct means to couple cell proliferation with biosynthesis of the translation machinery and the rate of protein synthesis.