| |  Online-Ressource |
|---|
| Verfasst von: | Viol, Linda [VerfasserIn]  |
|---|
| | Hata, Shoji [VerfasserIn] |
|---|
| | Pastor Peidro, Ana [VerfasserIn] |
|---|
| | Neuner, Annett [VerfasserIn] |
|---|
| | Murke, Florian [VerfasserIn] |
|---|
| | Wuchter, Patrick [VerfasserIn] |
|---|
| | Ho, Anthony Dick [VerfasserIn] |
|---|
| | Giebel, Bernd [VerfasserIn] |
|---|
| | Pereira, Gislene [VerfasserIn] |
|---|
| Titel: | Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis |
|---|
| Verf.angabe: | Linda Viol, Shoji Hata, Ana Pastor-Peidro, Annett Neuner, Florian Murke, Patrick Wuchter, Anthony D. Ho, Bernd Giebel, and Gislene Pereira |
|---|
| E-Jahr: | 2020 |
|---|
| Jahr: | February 14 2020 |
|---|
| Umfang: | 28 S. |
|---|
| Fussnoten: | Gesehen am 17.12.2020 |
|---|
| Titel Quelle: | Enthalten in: The journal of cell biology |
|---|
| Ort Quelle: | New York, NY : Rockefeller Univ. Press, 1962 |
|---|
| Jahr Quelle: | 2020 |
|---|
| Band/Heft Quelle: | 219(2020,3) Artikel-Nummer e201907136, 28 Seiten |
|---|
| ISSN Quelle: | 1540-8140 |
|---|
| Abstract: | Distal appendages (DAs) of the mother centriole are essential for the initial steps of ciliogenesis in G1/G0 phase of the cell cycle. DAs are released from centrosomes in mitosis by an undefined mechanism. Here, we show that specific DAs lose their centrosomal localization at the G2/M transition in a manner that relies upon Nek2 kinase activity to ensure low DA levels at mitotic centrosomes. Overexpression of active Nek2A, but not kinase-dead Nek2A, prematurely displaced DAs from the interphase centrosomes of immortalized retina pigment epithelial (RPE1) cells. This dramatic impact was also observed in mammary epithelial cells with constitutively high levels of Nek2. Conversely, Nek2 knockout led to incomplete dissociation of DAs and cilia in mitosis. As a consequence, we observed the presence of a cilia remnant that promoted the asymmetric inheritance of ciliary signaling components and supported cilium reassembly after cell division. Together, our data establish Nek2 as an important kinase that regulates DAs before mitosis. |
|---|
| DOI: | doi:10.1083/jcb.201907136 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1083/jcb.201907136 |
|---|
| | DOI: https://doi.org/10.1083/jcb.201907136 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| K10plus-PPN: | 1743138067 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis / Viol, Linda [VerfasserIn]; February 14 2020 (Online-Ressource)
