| |  Online-Ressource |
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| Verfasst von: | Cortese, Mirko [VerfasserIn]  |
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| | Lee, Ji Young [VerfasserIn] |
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| | Cerikan, Berati [VerfasserIn] |
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| | Neufeldt, Christopher [VerfasserIn] |
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| | Oorschot, Viola M. J. [VerfasserIn] |
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| | Köhrer, Sebastian [VerfasserIn] |
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| | Hennies, Julian [VerfasserIn] |
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| | Schieber, Nicole L. [VerfasserIn] |
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| | Ronchi, Paolo [VerfasserIn] |
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| | Mizzon, Giulia [VerfasserIn] |
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| | Romero-Brey, Inés [VerfasserIn] |
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| | Santarella-Mellwig, Rachel [VerfasserIn] |
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| | Schorb, Martin [VerfasserIn] |
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| | Boermel, Mandy [VerfasserIn] |
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| | Mocaer, Karel [VerfasserIn] |
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| | Beckwith, Marianne S. [VerfasserIn] |
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| | Templin, Rachel M. [VerfasserIn] |
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| | Gross, Viktoriia [VerfasserIn] |
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| | Pape, Constantin [VerfasserIn] |
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| | Tischer, Christian [VerfasserIn] |
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| | Frankish, Jamie [VerfasserIn] |
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| | Horvat, Natalie [VerfasserIn] |
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| | Laketa, Vibor [VerfasserIn] |
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| | Stanifer, Megan [VerfasserIn] |
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| | Boulant, Steeve [VerfasserIn] |
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| | Ruggieri, Alessia [VerfasserIn] |
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| | Chatel-Chaix, Laurent [VerfasserIn] |
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| | Schwab, Yannick [VerfasserIn] |
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| | Bartenschlager, Ralf [VerfasserIn] |
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| Titel: | Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies |
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| Verf.angabe: | Mirko Cortese, Ji-Young Lee, Berati Cerikan, Christopher J. Neufeldt, Viola M. J. Oorschot, Sebastian Köhrer, Julian Hennies, Nicole L. Schieber, Paolo Ronchi, Giulia Mizzon, Inés Romero-Brey, Rachel Santarella-Mellwig, Martin Schorb, Mandy Boermel, Karel Mocaer, Marianne S. Beckwith, Rachel M. Templin, Viktoriia Gross, Constantin Pape, Christian Tischer, Jamie Frankish, Natalie K. Horvat, Vibor Laketa, Megan Stanifer, Steeve Boulant, Alessia Ruggieri, Laurent Chatel-Chaix, Yannick Schwab, and Ralf Bartenschlager |
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| E-Jahr: | 2020 |
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| Jahr: | November 17, 2020 |
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| Umfang: | 20 S. |
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| Fussnoten: | Gesehen am 12.01.2021 |
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| Titel Quelle: | Enthalten in: Cell host and microbe |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 2007 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 28(2020), 6, Seite 853-866.e5 |
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| ISSN Quelle: | 1934-6069 |
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| Abstract: | Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation-dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2-infected human lung epithelial cells. We report 3D electron microscopy reconstructions of whole cells and subcellular compartments, revealing extensive fragmentation of the Golgi apparatus, alteration of the mitochondrial network and recruitment of peroxisomes to viral replication organelles formed by clusters of double-membrane vesicles (DMVs). These are tethered to the endoplasmic reticulum, providing insights into DMV biogenesis and spatial coordination of SARS-CoV-2 replication. Live cell imaging combined with an infection sensor reveals profound remodeling of cytoskeleton elements. Pharmacological inhibition of their dynamics suppresses SARS-CoV-2 replication. We thus report insights into virus-induced cytopathic effects and provide alongside a comprehensive publicly available repository of 3D datasets of SARS-CoV-2-infected cells for download and smooth online visualization. |
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| DOI: | doi:10.1016/j.chom.2020.11.003 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.chom.2020.11.003 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S193131282030620X |
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| | DOI: https://doi.org/10.1016/j.chom.2020.11.003 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | coronavirus |
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| | cytoskeleton |
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| | electron tomography |
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| | FIB-SEM |
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| | Golgi |
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| | intermediate filaments |
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| | live cell imaging |
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| | membrane remodeling |
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| | peroxisomes |
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| | viral replication organelles |
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| K10plus-PPN: | 1744400113 |
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| Verknüpfungen: | → Zeitschrift |
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Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies / Cortese, Mirko [VerfasserIn]; November 17, 2020 (Online-Ressource)
