Moderate G6PD deficiency increases mutation rates in the brain of mice

• Verfasst von: Felix, Klaus M. [VerfasserIn]
• Verfasst von: Nair, Jagadeesan [VerfasserIn]
• Verfasst von: Bartsch, Helmut [VerfasserIn]
• Titel: Moderate G6PD deficiency increases mutation rates in the brain of mice
• Verf.angabe: Klaus Felix, Lynne D Rockwood, Walter Pretsch, Jagadeesan Nair, Helmut Bartsch, Georg-Wilhelm Bornkamm, and Siegfried Janz
• E-Jahr: 2002
• Jahr: 14 March 2002
• Umfang: 11 S.
• Fussnoten: Gesehen am 12.01.2021
• Titel Quelle: Enthalten in: Free radical biology and medicine
• Ort Quelle: New York, NY [u.a.] : Elsevier, 1987
• Jahr Quelle: 2002
• Band/Heft Quelle: 32(2002), 7, Seite 663-673
• ISSN Quelle: 1873-4596
• DOI: doi:10.1016/S0891-5849(02)00756-6
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Endogenous oxidative stress
• Sach-SW: Etheno DNA adducts
• Sach-SW: Free radicals
• Sach-SW: Glucose-6-phoshpate dehydrogenase
• Sach-SW: In vivo mutagenicity reporter gene
• Sach-SW: Z
• K10plus-PPN: 1744421374

Abstract

Mice that harbored the x-ray-induced low efficiency allele of the major X-linked isozyme of glucose-6-phospate dehydrogenase (G6PD), Gpdxa-m2Neu, and, in addition, harbored the transgenic shuttle vector for the determination of mutagenesis in vivo, pUR288, were employed to further our understanding of the interdependence of general metabolism, oxidative stress control, and somatic mutagenesis. The Gpdxa-m2Neu mutation conferred moderate G6PD deficiency in hemizygous males (Gpdxa-m2Neu/y) displaying residual enzyme activities of 27% in red blood cells and 13% in brain (compared to wild-type controls, Gpdxa/y males). In spite of this mild phenotype, the brains of G6PD-deficient males exhibited a significant distortion of redox control (∼3-fold decrease in the ratio of reduced glutathione to oxidized glutathione), a considerable accumulation of promutagenic etheno DNA adducts (∼13-fold increase in ethenodeoxyadenosine and ∼5-fold increase in ethenodeoxycytidine), and a substantial elevation of somatic mutation rates (∼3-fold increase in mutant frequencies in lacZ, the target and reporter gene of mutagenesis in the shuttle vector, pUR288). The mutation pattern in the brain was dominated by illegitimate genetic recombinations, a presumed hallmark of oxidative mutagenesis. These findings suggested a critical function for G6PD in limiting oxidative mutagenesis in the mouse brain.