Growth hormone receptor abundance in tibial growth plates of uremic rats

• Verfasst von: Edmondson, Stephanie R. [VerfasserIn]
• Verfasst von: Baker, Naomi L. [VerfasserIn]
• Verfasst von: Oh, Jun [VerfasserIn]
• Verfasst von: Kovacs, Gabor [VerfasserIn]
• Verfasst von: Werther, George A. [VerfasserIn]
• Verfasst von: Mehls, Otto [VerfasserIn]
• Titel: Growth hormone receptor abundance in tibial growth plates of uremic rats
• Titelzusatz: GH/IGF-I treatment
• Verf.angabe: Stephanie R. Edmondson, Naomi L. Baker, Jun Oh, Gabor Kovacs, George A. Werther, Otto Mehls
• E-Jahr: 2015
• Jahr: 16 December 2015
• Jahr des Originals: 2000
• Umfang: 9 S.
• Teil: volume:58
• Teil: year:2000
• Teil: number:1
• Teil: pages:62-70
• Teil: extent:9
• Fussnoten: Available online 16 December 2015 ; Gesehen am 12.01.2021
• Titel Quelle: Enthalten in: Kidney international
• Ort Quelle: New York, NY : Elsevier, 1972
• Jahr Quelle: 2000
• Band/Heft Quelle: 58(2000), 1, Seite 62-70
• ISSN Quelle: 1523-1755
• DOI: doi:10.1046/j.1523-1755.2000.00141.x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: bone growth
• Sach-SW: chronic renal failure
• Sach-SW: development
• Sach-SW: epiphyseal growth plate
• Sach-SW: growth hormone
• Sach-SW: growth retardation
• Sach-SW: hypertrophy
• Sach-SW: insulin-like growth factor-I
• K10plus-PPN: 1744427232

Abstract

Growth hormone receptor abundance in tibial growth plates of uremic rats: GH/IGF-I treatment. - Background - Children with chronic renal failure (CRF) exhibit growth retardation and a disturbed growth hormone/insulin-like growth factor-I (GH/IGF-I) axis. Treatment of children with CRF with GH or GH/IGF-I can partially restore linear growth. The molecular basis for decreased longitudinal growth is not known but may involve an impaired action of GH. - Methods - We used the growth-retarded uremic rat model to determine the abundance and distribution of GH receptors (GHRs) in the tibial epiphyseal growth plate and the influence of GH, IGF-I, or combined GH/IGF-I treatment. Pair-fed rats were used as the control. - Results - While all treatment regimes increased body length and weight in both rat groups, only GH/IGF-I treatment increased the total growth plate width. This involved an increase in cell number in the hypertrophic zone, which could also be induced by IGF-I alone. Immunohistochemical analysis showed that uremic rats had decreased abundance of GHRs in the proliferative zone, and only GH/IGF-I therapy could overcome this decrease. These data thus suggest that growth retardation in uremic rats is, at least in part, due to a decrease in GHR abundance in chondrocytes of the proliferative zone of the tibial growth plate. This decreased GHR abundance can be overcome by combined GH/IGF-I therapy, thus enhancing generation and proliferation of hypertrophic zone chondrocytes and increasing growth-plate width. - Conclusion - These studies point to a mechanism for the growth retardation seen in children with CRF, and suggest that combined GH/IGF-I treatment may provide more effective therapy for these patients than GH alone.