| |  Online-Ressource |
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| Verfasst von: | Huang, Xiaojia [VerfasserIn]  |
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| | Luo, Wei [VerfasserIn] |
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| | Wu, Silin [VerfasserIn] |
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| | Long, Yuming [VerfasserIn] |
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| | Li, Rui [VerfasserIn] |
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| | Zheng, Fenghua [VerfasserIn] |
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| | Greiner, Steffen [VerfasserIn] |
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| | Rausch, Thomas [VerfasserIn] |
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| | Zhao, Hongbo [VerfasserIn] |
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| Titel: | Apoplastic maize fructan exohydrolase Zm-6-FEH displays substrate specificity for levan and is induced by exposure to levan-producing bacteria |
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| Verf.angabe: | Xiaojia Huang, Wei Luo, Silin Wu, Yuming Long, Rui Li, Fenghua Zheng, Steffen Greiner, Thomas Rausch, Hongbo Zhao |
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| E-Jahr: | 2020 |
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| Jahr: | 3 July 2020 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 14.01.2021 |
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| Titel Quelle: | Enthalten in: International journal of biological macromolecules |
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| Ort Quelle: | New York, NY [u.a.] : Elsevier, 1979 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 163(2020), Seite 630-639 |
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| ISSN Quelle: | 1879-0003 |
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| Abstract: | Fructan exohydrolases (FEHs) are structurally related to cell wall invertases. While the latter are ubiquitous in higher plants, the role of FEHs in non-fructan species has remained enigmatic. To explore possible roles of FEHs in maize, a full length putative Zm-6-FEH-encoding cDNA was cloned displaying high sequence similarity with cell wall invertases. For functional characterization, Zm-6-FEH protein was expressed in Picha pastoris and in Nicotiana benthamiana leaves. Enzyme activity of recombinant Zm-6-FEH protein showed a strong preference for levan as substrate. Expression profiling in maize seedlings revealed higher transcript amounts in the more mature leaf parts as compared to the growth zone at the base of the leaf, in good correlation with FEH enzyme activities. Subcellular localization analysis indicated Zm-6-FEH location in the apoplast. Noteworthy, incubation of leaf discs with levan and co-incubation with high levan-producing bacteria selectively up-regulated transcript levels of Zm-6-FEH, accompanied by an increase of 6-FEH enzyme activity. In summary, the results indicate that Zm-6-FEH, a novel fructan exohydrolase of a non-fructan species, may have a role in plant defense against levan-producing bacteria. |
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| DOI: | doi:10.1016/j.ijbiomac.2020.06.254 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.ijbiomac.2020.06.254 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0141813020337077 |
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| | DOI: https://doi.org/10.1016/j.ijbiomac.2020.06.254 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Abiotic stress |
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| | Fructan exohydrolase |
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| | Levan |
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| K10plus-PPN: | 174466143X |
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| Verknüpfungen: | → Zeitschrift |
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Apoplastic maize fructan exohydrolase Zm-6-FEH displays substrate specificity for levan and is induced by exposure to levan-producing bacteria / Huang, Xiaojia [VerfasserIn]; 3 July 2020 (Online-Ressource)
