Imaging dynamic redox processes with genetically encoded probes

• Verfasst von: Ezeriņa, Daria [VerfasserIn]
• Verfasst von: Morgan, Bruce [VerfasserIn]
• Verfasst von: Dick, Tobias P. [VerfasserIn]
• Titel: Imaging dynamic redox processes with genetically encoded probes
• Verf.angabe: Daria Ezeriņa, Bruce Morgan, Tobias P. Dick
• E-Jahr: 2014
• Jahr: 7 January 2014
• Umfang: 7 S.
• Fussnoten: Gesehen am 14.01.2021
• Titel Quelle: Enthalten in: Journal of molecular and cellular cardiology
• Ort Quelle: New York, NY [u.a.] : Elsevier, 1970
• Jahr Quelle: 2014
• Band/Heft Quelle: 73(2014), Seite 43-49
• ISSN Quelle: 1095-8584
• DOI: doi:10.1016/j.yjmcc.2013.12.023
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cpYFP
• Sach-SW: Genetically encoded redox probes
• Sach-SW: Hydrogen peroxide
• Sach-SW: roGFP
• Sach-SW: Superoxide
• K10plus-PPN: 1744694125

Abstract

Redox signalling plays an important role in many aspects of physiology, including that of the cardiovascular system. Perturbed redox regulation has been associated with numerous pathological conditions; nevertheless, the causal relationships between redox changes and pathology often remain unclear. Redox signalling involves the production of specific redox species at specific times in specific locations. However, until recently, the study of these processes has been impeded by a lack of appropriate tools and methodologies that afford the necessary redox species specificity and spatiotemporal resolution. Recently developed genetically encoded fluorescent redox probes now allow dynamic real-time measurements, of defined redox species, with subcellular compartment resolution, in intact living cells. Here we discuss the available genetically encoded redox probes in terms of their sensitivity and specificity and highlight where uncertainties or controversies currently exist. Furthermore, we outline major goals for future probe development and describe how progress in imaging methodologies will improve our ability to employ genetically encoded redox probes in a wide range of situations. This article is part of a special issue entitled “Redox Signalling in the Cardiovascular System.”