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Verfasst von:Grau, Nicole [VerfasserIn]   i
 Scherer, Sandra [VerfasserIn]   i
 Rothley, Melanie [VerfasserIn]   i
 Roßwag, Sven [VerfasserIn]   i
 Thiele, Wilko [VerfasserIn]   i
 Stoecklein, Nikolas H. [VerfasserIn]   i
 Cremers, Natascha [VerfasserIn]   i
 Thaler, Sonja [VerfasserIn]   i
 Garvalov, Boyan K. [VerfasserIn]   i
 Sleeman, Jonathan P. [VerfasserIn]   i
Titel:Spatiotemporally controlled induction of gene expression in vivo allows tracking the fate of tumor cells that traffic through the lymphatics
Verf.angabe:Nicole Grau, Sandra D. Scherer, Melanie Rothley, Sven Roßwag, Wilko Thiele, Nikolas H. Stoecklein, Natascha Cremers, Sonja Thaler, Boyan K. Garvalov and Jonathan P. Sleeman
Jahr:2020
Jahr des Originals:2019
Umfang:9 S.
Fussnoten:First published: 01 November 2019 ; Gesehen am 19.01.2021
Titel Quelle:Enthalten in: International journal of cancer
Ort Quelle:Bognor Regis : Wiley-Liss, 1966
Jahr Quelle:2020
Band/Heft Quelle:147(2020), 4, Seite 1190-1198
ISSN Quelle:1097-0215
Abstract:Metastasis is a multistep process, during which circulating tumor cells traffic through diverse anatomical locations. Stable inducible marking of tumor cells in a manner that is tightly spatially and temporally controlled would allow tracking the contribution of cells passing through specific locations to metastatic dissemination. For example, tumor cells enter the lymphatic system and can form metastases in regional lymph nodes, but the relative contribution of tumor cells that traffic through the lymphatic system to the formation of distant metastases remains controversial. Here, we developed a novel genetic switch based on mild transient warming (TW) that allows cells to be marked in a defined spatiotemporal manner in vivo. Prior to warming, cells express only EGFP. Upon TW, the EGFP gene is excised and expression of mCherry is permanently turned on. We employed this system in an experimental pancreatic cancer model and used localized TW to induce the genetic switch in tumor cells trafficking through tumor-draining lymph nodes. Thereby we found that tumor cells disseminating via the lymphatics make a major contribution to the seeding of lung metastases. The inducible genetic marking system we have developed is a powerful tool for the tracking of metastasizing cells in vivo.
DOI:doi:10.1002/ijc.32766
URL:Volltext: https://doi.org/https://doi.org/10.1002/ijc.32766
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.32766
 DOI: https://doi.org/10.1002/ijc.32766
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:cell tracking
 genetic switch
 heat-inducible expression
 lymph node
 metastasis
K10plus-PPN:1744989648
Verknüpfungen:→ Zeitschrift
 
 
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