Rapid response to pandemic threats

• Verfasst von: Heiß, Kirsten [VerfasserIn]
• Verfasst von: Heidepriem, Jasmin [VerfasserIn]
• Verfasst von: Fischer, Nico [VerfasserIn]
• Verfasst von: Weber, Laura [VerfasserIn]
• Verfasst von: Dahlke, Christine [VerfasserIn]
• Verfasst von: Jänisch, Thomas [VerfasserIn]
• Verfasst von: Löffler, Felix [VerfasserIn]
• Titel: Rapid response to pandemic threats
• Titelzusatz: immunogenic epitope detection of pandemic pathogens for diagnostics and vaccine development using peptide microarrays
• Verf.angabe: Kirsten Heiss, Jasmin Heidepriem, Nico Fischer, Laura K. Weber, Christine Dahlke, Thomas Jaenisch, Felix F. Loeffler
• E-Jahr: 2020
• Jahr: September 5, 2020
• Umfang: 16 S.
• Fussnoten: Gesehen am 20.01.2021
• Titel Quelle: Enthalten in: Journal of proteome research
• Ort Quelle: Washington, DC : ACS Publications, 2002
• Jahr Quelle: 2020
• Band/Heft Quelle: 19(2020), 11, Seite 4339-4354
• ISSN Quelle: 1535-3907
• DOI: doi:10.1021/acs.jproteome.0c00484
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1745067809

Abstract

Emergence and re-emergence of pathogens bearing the risk of becoming a pandemic threat are on the rise. Increased travel and trade, growing population density, changes in urbanization, and climate have a critical impact on infectious disease spread. Currently, the world is confronted with the emergence of a novel coronavirus SARS-CoV-2, responsible for yet more than 800000 deaths globally. Outbreaks caused by viruses, such as SARS-CoV-2, HIV, Ebola, influenza, and Zika, have increased over the past decade, underlining the need for a rapid development of diagnostics and vaccines. Hence, the rational identification of biomarkers for diagnostic measures on the one hand, and antigenic targets for vaccine development on the other, are of utmost importance. Peptide microarrays can display large numbers of putative target proteins translated into overlapping linear (and cyclic) peptides for a multiplexed, high-throughput antibody analysis. This enabled for example the identification of discriminant/diagnostic epitopes in Zika or influenza and mapping epitope evolution in natural infections versus vaccinations. In this review, we highlight synthesis platforms that facilitate fast and flexible generation of high-density peptide microarrays. We further outline the multifaceted applications of these peptide array platforms for the development of serological tests and vaccines to quickly encounter pandemic threats.