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Verfasst von:Ringkowski, Sabine [VerfasserIn]   i
Titel:Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis
Mitwirkende:Herth, Felix   i
Verf.angabe:Sabine Ringkowski, Joshua Loke, Shuying Huang, Hasib Ahmadzai, Felix J.F. Herth, Paul S. Thomas, Cristan Herbert
E-Jahr:2016
Jahr:1 July 2016
Umfang:11 S.
Fussnoten:Gesehen am 21.01.2021
Titel Quelle:Enthalten in: Respiratory medicine
Ort Quelle:Burlington, Mass. : Harcourt, 1999
Jahr Quelle:2016
Band/Heft Quelle:117(2016), Seite 243-253
ISSN Quelle:1532-3064
Abstract:Methods: Using a cross-sectional study design, plasma and peripheral blood mononuclear cells (PBMC) were collected from patients and control subjects. Protein and mRNA (in PBMC) levels for IL-27 and IL-35 (IL27, EBI3, IL12A subunits) as well as IL-27 receptor (IL6ST and IL27RA subunits) were assessed spontaneously and following direct (LPS) and indirect (anti-CD3/28 activation beads) macrophage stimulation using RT- PCR, ELISA and flow cytometry. - Results: Following stimulation with LPS, PBMC of patients with sarcoidosis displayed significantly enhanced expression of IL27 and EBI3 mRNA (p ¼ 0.020 and p ¼ 0.037 respectively) compared to PBMCs from healthy controls. There was also significantly enhanced production of IL-27 by PBMC from patients with sarcoidosis compared to healthy controls in response to LPS stimulation (p ¼ 0.027). IL6ST mRNA and IL6ST protein were significantly lower in patients with sarcoidosis (mRNA p ¼ 0.0002; MFI p ¼ 0.0015) whilst IL27RA protein levels were significantly higher in patients with sarcoidosis compared to healthy controls (MFI p < 0.0001). Plasma IL-35 protein levels did not differ between control and sarcoidosis subjects (p ¼ 0.23). - Conclusion: These results suggest there may be exaggerated activation of IL-27 signalling in response to LPS in sarcoidosis.
DOI:doi:10.1016/j.rmed.2016.06.021
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.rmed.2016.06.021
 Volltext: https://linkinghub.elsevier.com/retrieve/pii/S0954611116301470
 DOI: https://doi.org/10.1016/j.rmed.2016.06.021
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:174514563X
Verknüpfungen:→ Zeitschrift

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