Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis

• Verfasst von: Ringkowski, Sabine [VerfasserIn]
• Titel: Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis
• Mitwirkende: Herth, Felix
• Verf.angabe: Sabine Ringkowski, Joshua Loke, Shuying Huang, Hasib Ahmadzai, Felix J.F. Herth, Paul S. Thomas, Cristan Herbert
• E-Jahr: 2016
• Jahr: 1 July 2016
• Umfang: 11 S.
• Fussnoten: Gesehen am 21.01.2021
• Titel Quelle: Enthalten in: Respiratory medicine
• Ort Quelle: Burlington, Mass. : Harcourt, 1999
• Jahr Quelle: 2016
• Band/Heft Quelle: 117(2016), Seite 243-253
• ISSN Quelle: 1532-3064
• DOI: doi:10.1016/j.rmed.2016.06.021
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 174514563X

Abstract

Methods: Using a cross-sectional study design, plasma and peripheral blood mononuclear cells (PBMC) were collected from patients and control subjects. Protein and mRNA (in PBMC) levels for IL-27 and IL-35 (IL27, EBI3, IL12A subunits) as well as IL-27 receptor (IL6ST and IL27RA subunits) were assessed spontaneously and following direct (LPS) and indirect (anti-CD3/28 activation beads) macrophage stimulation using RT- PCR, ELISA and flow cytometry. - Results: Following stimulation with LPS, PBMC of patients with sarcoidosis displayed significantly enhanced expression of IL27 and EBI3 mRNA (p ¼ 0.020 and p ¼ 0.037 respectively) compared to PBMCs from healthy controls. There was also significantly enhanced production of IL-27 by PBMC from patients with sarcoidosis compared to healthy controls in response to LPS stimulation (p ¼ 0.027). IL6ST mRNA and IL6ST protein were significantly lower in patients with sarcoidosis (mRNA p ¼ 0.0002; MFI p ¼ 0.0015) whilst IL27RA protein levels were significantly higher in patients with sarcoidosis compared to healthy controls (MFI p < 0.0001). Plasma IL-35 protein levels did not differ between control and sarcoidosis subjects (p ¼ 0.23). - Conclusion: These results suggest there may be exaggerated activation of IL-27 signalling in response to LPS in sarcoidosis.