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Verfasst von:Rinaldetti, Sébastien [VerfasserIn]   i
 Worst, Thomas [VerfasserIn]   i
 Rempel, Eugen [VerfasserIn]   i
 Kriegmair, Maximilian [VerfasserIn]   i
 Hartmann, Arndt [VerfasserIn]   i
 Porubsky, Stefan [VerfasserIn]   i
 Bolenz, Christian [VerfasserIn]   i
 Erben, Philipp [VerfasserIn]   i
Titel:Subtype specific expression and survival prediction of pivotal lncRNAs in muscle invasive bladder cancer
Verf.angabe:Sebastien Rinaldetti, Thomas Stefan Worst, Eugen Rempel, Maximilian C. Kriegmair, Arndt Hartmann, Stefan Porubsky, Christian Bolenz & Philipp Erben
E-Jahr:2020
Jahr:24 November 2020
Umfang:9 S.
Fussnoten:Gesehen am 28.01.2021
Titel Quelle:Enthalten in: Scientific reports
Ort Quelle:[London] : Springer Nature, 2011
Jahr Quelle:2020
Band/Heft Quelle:10(2020) Artikel-Nummer 20472, 9 Seiten
ISSN Quelle:2045-2322
Abstract:Comprehensive transcriptome expression analyses of bladder cancer revealed distinct lncRNA clusters with differential molecular and clinical characteristics. In this study, pivotal lncRNAs were assessed for their impact on survival and their differential expression between the molecular bladder cancer subtypes. FFPE samples from chemotherapy-naïve patients with muscle invasive bladder cancer (MIBC) were analyzed on the Nanostring nCounter platform for absolute quantification. An established 36-gene panel was used for molecular subtype classification into basal, luminal and infiltrated MIBC. In a second step, 14 pivotal lncRNAs were assessed for their molecular subtype attribution, and their predictive value in disease-specific survival. In silico validation was performed on a total of 487 MIBC patients (MDA, TGCA and Chungbuk cohort). Several pivotal lncRNAs showed a distinct molecular subtype attribution: e.g. MALAT1 showed a downregulation in the basal subtype (p = 0.009), TUG1 and CBR3AS1 showed an upregulation in the luminal subtype (p ≤ 0.001). High transcript levels of SNHG16, CBR3AS1 and H19 appeared to be predictive for a shorter disease-specific survival. Patients overexpressing putative oncogenes MALAT1 and TUG1 in MIBC tissue presented prolonged survival, suggesting tumor suppressive effects of both lncRNAs. The Nanostring nCounter proved to be a valid platform for the quantification of low-abundance transcripts including lncRNAs.
DOI:doi:10.1038/s41598-020-77252-2
URL:kostenfrei: Volltext: https://doi.org/10.1038/s41598-020-77252-2
 kostenfrei: Volltext: https://www.nature.com/articles/s41598-020-77252-2
 DOI: https://doi.org/10.1038/s41598-020-77252-2
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1745880933
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