New DNA-based biomarkers for oxidative stress and cancer chemoprevention studies

• Verfasst von: Bartsch, Helmut [VerfasserIn]
• Verfasst von: Nair, Jagadeesan [VerfasserIn]
• Titel: New DNA-based biomarkers for oxidative stress and cancer chemoprevention studies
• Verf.angabe: H. Bartsch, J. Nair
• E-Jahr: 2000
• Jahr: 5 July 2000
• Umfang: 6 S.
• Fussnoten: Gesehen am 28.01.2000
• Titel Quelle: Enthalten in: European journal of cancer
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 1965
• Jahr Quelle: 2000
• Band/Heft Quelle: 36(2000), 10, Seite 1229-1234
• ISSN Quelle: 1879-0852
• DOI: doi:10.1016/S0959-8049(00)00095-2
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Biomarkers
• Sach-SW: Cancer prevention
• Sach-SW: Etheno-DNA adducts
• Sach-SW: Lipid peroxidation
• Sach-SW: Oxidative stress
• K10plus-PPN: 1745929533

Abstract

Etheno (ε) modified DNA bases (εdA, εdC, εdG) are generated from the carcinogens vinyl chloride and urethane, but also by reactions of DNA with products derived from lipid peroxidation (LPO) and oxidative stress via endogenous pathways. Recently developed ultrasensitive methods allowed the detection of these ε-adducts in vivo and their role to be studied in experimental and human carcinogenesis. Highly variable background levels of ε-adducts were detected in DNA from different organs of unexposed humans and rodents. Several known cancer risk factors increased the level of these DNA lesions in target organs: elevated ε-adducts were found in hepatic DNA from patients with metal storage diseases, after overproduction of nitric oxide (NO) by inducible NO synthase (iNOS) in a mouse model, and in colonic polyps of familial adenomatous polyposis patients. A high ω-6-polyunsaturated fatty acid diet increased ε-DNA adducts in white blood cells of female subjects. In conclusion, ε-adducts were found to be elevated in cancer-prone tissues, suggesting that these promutagenic lesions could drive cells to malignancy. Therefore, exocyclic DNA adducts offer new tools in cancer aetiological research and in verifying the efficacy of chemopreventive agents.