Multiple random phosphorylations in clock proteins provide long delays and switches

• Verfasst von: Upadhyay, Abhishek [VerfasserIn]
• Verfasst von: Marzoll, Daniela [VerfasserIn]
• Verfasst von: Diernfellner, Axel [VerfasserIn]
• Verfasst von: Brunner, Michael [VerfasserIn]
• Verfasst von: Herzel, Hanspeter [VerfasserIn]
• Titel: Multiple random phosphorylations in clock proteins provide long delays and switches
• Verf.angabe: Abhishek Upadhyay, Daniela Marzoll, Axel Diernfellner, Michael Brunner & Hanspeter Herzel
• E-Jahr: 2020
• Jahr: 17 December 2020
• Umfang: 13 S.
• Fussnoten: Gesehen am 01.02.2021
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2020
• Band/Heft Quelle: 10(2020) Artikel-Nummer 22224, 13 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/s41598-020-79277-z
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 174632015X

Abstract

Theory predicts that self-sustained oscillations require robust delays and nonlinearities (ultrasensitivity). Delayed negative feedback loops with switch-like inhibition of transcription constitute the core of eukaryotic circadian clocks. The kinetics of core clock proteins such as PER2 in mammals and FRQ in Neurospora crassa is governed by multiple phosphorylations. We investigate how multiple, slow and random phosphorylations control delay and molecular switches. We model phosphorylations of intrinsically disordered clock proteins (IDPs) using conceptual models of sequential and distributive phosphorylations. Our models help to understand the underlying mechanisms leading to delays and ultrasensitivity. The model shows temporal and steady state switches for the free kinase and the phosphoprotein. We show that random phosphorylations and sequestration mechanisms allow high Hill coefficients required for self-sustained oscillations.