Redox-mediated functional and structural changes in insulin receptor kinase

• Verfasst von: Hotz-Wagenblatt, Agnes [VerfasserIn]
• Verfasst von: Dröge, Wulf [VerfasserIn]
• Titel: Redox-mediated functional and structural changes in insulin receptor kinase
• Verf.angabe: by Agnes Hotz-Wagenblatt anf Wulf Dröge
• Jahr: 2002
• Umfang: 9 S.
• Fussnoten: Gesehen am 02.02.2021
• Titel Quelle: Enthalten in: Protein sensors and reactive oxygen species ; B: Thiol enzymes and proteins
• Ort Quelle: San Diego] : Academic Press, 2002
• Jahr Quelle: 2002
• Band/Heft Quelle: (2002), Seite 288-296
• ISBN Quelle: 978-0-12-182251-4
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1746738588

Abstract

This chapter describes the methods used to demonstrate redox effects on the structure and function of the insuling receptor kinase (IRK). Under these methods, the chapter describes an experimental protocol showing that treatment of intact Chinese hamster ovary (CHO)-HIR cells with low micromolar hydrogen) enhances tyrosine phosphorylation in response to insulin. The second method is the method of molecular modeling by energy minimization, which predicts moderate structural changes because of slight posttranslational modifications of an already folded protein. The other methods include three-dimensional modeling by energy minimization, identification of permissive and nonpermissive steps in sequential posttranslational modification of IRK domain, insertion of adenylyl imidodiphosphate (AMP-PNP) into ATP-binding site.