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Verfasst von:Haselmann, Verena [VerfasserIn]   i
 Kittel, Maximilian [VerfasserIn]   i
 Gerhards, Catharina [VerfasserIn]   i
 Thiaucourt, Margot [VerfasserIn]   i
 Eichner, Romy [VerfasserIn]   i
 Costina, Victor [VerfasserIn]   i
 Neumaier, Michael [VerfasserIn]   i
Titel:Comparison of test performance of commercial anti-SARS-CoV-2 immunoassays in serum and plasma samples
Verf.angabe:Verena Haselmann, Maximilian Kittel, Catharina Gerhards, Margot Thiaucourt, Romy Eichner, Victor Costina, Michael Neumaier
E-Jahr:2020
Jahr:9 July 2020
Umfang:6 S.
Fussnoten:Gesehen am 03.02.2021
Titel Quelle:Enthalten in: Clinica chimica acta
Ort Quelle:Amsterdam [u.a.] : Elsevier Science, 1956
Jahr Quelle:2020
Band/Heft Quelle:510(2020), Seite 73-78
ISSN Quelle:1873-3492
Abstract:Background - For epidemiologic, social and economic reasons, assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection prevalence and immunity are important to adapt decisions to current demands. Hence, immunoassays for detection of anti-SARS-CoV-2 antibodies are introduced rapidly without requiring FDA emergency use authorization approval. Thus, evaluation of test performance predominantly relies on laboratories. This study aimed to evaluate the test performance of recently launched commercial immunoassays in serum and plasma samples. - Methods - 51 serum samples from 26 patients with confirmed SARS-CoV-2 infection after end of quarantine and 25 control patients were analyzed using anti-SARS-CoV-2 IgG immunoassays from Roche, Euroimmun and Epitope to assess diagnostic sensitivity and specificity. 20 matching pairs of serum and plasma samples were included to analyze comparability between different specimens. - Results - Overall, a diagnostic sensitivity of 92.3%, 96.2-100% and 100% with a respective diagnostic specificity of 100%, 100% and 84-86% for the immunoassays from Roche, Euroimmun and Epitope were determined. In total, 84-96% of samples were correctly classified as negative and 92.3-95.2% as positive. The level of concordance between plasma- and serum-based testing diverged between the assays (Epitope r2 = 0.97; Euroimmun r2 = 0.91; Roche r2 = 0.76). - Conclusions - The immunoassays from Euroimmun and Roche revealed a higher specificity than the Epitope assay without a substantial drop of diagnostic sensitivity. Significant differences between plasma- and serum-based testing highlights the need for determination of appropriate cut-offs per specimen type. Hence, there is an urgent need for test harmonization and establishment of quality standards for an appropriate use of COVID-19 serological tests.
DOI:doi:10.1016/j.cca.2020.07.007
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.cca.2020.07.007
 Volltext: http://www.sciencedirect.com/science/article/pii/S0009898120303235
 DOI: https://doi.org/10.1016/j.cca.2020.07.007
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Anti-SARS-CoV-2
 COVID-19
 Immunoassay
 Method comparison
 Roche
 Serologic assay
K10plus-PPN:1743813627
Verknüpfungen:→ Zeitschrift

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