The Drosophila proteins pelle and tube induce JNK/AP-1 activity in mammalian cells

• Verfasst von: Bacher, Susanne [VerfasserIn]
• Verfasst von: Dröge, Wulf [VerfasserIn]
• Titel: The Drosophila proteins pelle and tube induce JNK/AP-1 activity in mammalian cells
• Verf.angabe: Susanne Bacher, Jörg Großhans, Wulf Dröge, M. Lienhard Schmitz
• E-Jahr: 2001
• Jahr: 22 May 2001
• Umfang: 6 S.
• Fussnoten: First published online 10 May 2001 ; Gesehen am 03.02.2001
• Titel Quelle: Enthalten in: Vereinigung der Europäischen Biochemischen GesellschaftenFEBS letters
• Ort Quelle: Chichester : Wiley, 1968
• Jahr Quelle: 2001
• Band/Heft Quelle: 497(2001), 2-3, Seite 153-158
• ISSN Quelle: 1873-3468
• DOI: doi:10.1016/S0014-5793(01)02454-1
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: AP-1
• Sach-SW: c-Jun N-terminal kinase
• Sach-SW: Interleukin-1 signaling
• Sach-SW: Nuclear factor-κB
• Sach-SW: Pelle
• Sach-SW: Tube
• K10plus-PPN: 1747190721

Abstract

The mammalian interleukin-1 (IL-1) signal transduction pathways display remarkable homology to the Toll signaling cascade in Drosophila. To address the question whether members of the Drosophila Toll pathway are functional in mammalian cells, inactive and constitutively active versions of the protein kinase Pelle and its regulator Tube were expressed in HeLa cells and tested for their impact on IL-1-dependent signaling events. The Drosophila proteins failed to induce the IL-1-responsive transcription factor, nuclear factor-κB, but selectively activated the IL-1-regulated kinase, c-Jun N-terminal kinase (JNK), thus resulting in elevated AP-1 activity. Activation of JNK/AP-1 activity was seen upon expression of a Pelle mutant lacking its C-terminal half or by a membrane-bound and multimerised Tube protein, showing the functionality of the Drosophila proteins in mammalian cells.