Cell-type specific expression and regulation of apolipoprotein D and E in human endometrium

• Verfasst von: Germeyer, Ariane [VerfasserIn]
• Verfasst von: Capp, Edison [VerfasserIn]
• Verfasst von: Schlicksupp, Franziska Verena [VerfasserIn]
• Verfasst von: Jauckus, Julia [VerfasserIn]
• Verfasst von: von Rango, Ulrike [VerfasserIn]
• Verfasst von: Wolff, Michael von [VerfasserIn]
• Verfasst von: Strowitzki, Thomas [VerfasserIn]
• Titel: Cell-type specific expression and regulation of apolipoprotein D and E in human endometrium
• Verf.angabe: Ariane Germeyer, Edison Capp, Franziska Schlicksupp, Julia Jauckus, Ulrike von Rango, Michael von Wolff, Thomas Strowitzki
• E-Jahr: 2013
• Jahr: 26 July 2013
• Umfang: 5 S.
• Fussnoten: Gesehen am 04.02.2020
• Titel Quelle: Enthalten in: European journal of obstetrics & gynecology and reproductive biology
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1971
• Jahr Quelle: 2013
• Band/Heft Quelle: 170(2013), 2, Seite 487-491
• ISSN Quelle: 1872-7654
• DOI: doi:10.1016/j.ejogrb.2013.06.043
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Apolipoprotein D
• Sach-SW: Apolipoprotein E
• Sach-SW: Endometrium
• Sach-SW: Gene and protein expression
• K10plus-PPN: 1747396168

Abstract

Objective - To assess the expression and regulation of antilipoprotein D (ApoD) and antilipoprotein E (ApoE) in human endometrium. - Study design - Endometrial biopsies from healthy, regularly cycling women were collected during the late proliferative and mid-secretory phase. mRNA gene expression of ApoD and ApoE was determined using real-time PCR in whole tissue, in isolated stromal (ESC), epithelial (EEC) and CD45+ leukocytes (EIC), as well as after hormonal stimulation of ESC and EEC in vitro. Protein expression was analyzed using immunohistochemistry. - Results - ApoD and ApoE mRNA was expressed in all cell types examined. A rise in ApoD mRNA expression was seen in whole endometrium, ESC, and EEC in the secretory phase, as well as after hormonal stimulation of ESC and EEC in vitro. ApoE mRNA was significantly upregulated in whole endometrium of secretory phase biopsies, while its expression was not altered by progesterone in vitro. Immunohistochemistry of whole endometrial tissue localized ApoD mainly in ESC and EEC. While ApoE was localized slightly in ESC, it was particularly noted on the surface of secretory phase endothelial cells. - Conclusion - We demonstrate for the first time the cell-type and cycle dependent expression of ApoD and ApoE within human endometrium, suggesting their role in endometrial modulation.