| |  Online-Ressource |
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| Verfasst von: | Haag, Daniel [VerfasserIn]  |
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| | Mack, Norman [VerfasserIn] |
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| | Benites Goncalves da Silva, Patricia [VerfasserIn] |
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| | Statz, Britta [VerfasserIn] |
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| | Clark, Jessica [VerfasserIn] |
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| | Tanabe, Koji [VerfasserIn] |
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| | Sharma, Tanvi [VerfasserIn] |
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| | Jäger, Natalie [VerfasserIn] |
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| | Jones, David T. W. [VerfasserIn] |
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| | Kawauchi, Daisuke [VerfasserIn] |
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| | Wernig, Marius [VerfasserIn] |
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| | Pfister, Stefan [VerfasserIn] |
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| Titel: | H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model |
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| Verf.angabe: | Daniel Haag, Norman Mack, Patricia Benites Goncalves da Silva, Britta Statz, Jessica Clark, Koji Tanabe, Tanvi Sharma, Natalie Jäger, David T.W. Jones, Daisuke Kawauchi, Marius Wernig, and Stefan M. Pfister |
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| E-Jahr: | 2021 |
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| Jahr: | 4 February 2021 |
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| Umfang: | 29 S. |
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| Fussnoten: | Gesehen am 05.02.2021 |
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| Titel Quelle: | Enthalten in: Cancer cell |
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| Ort Quelle: | Cambridge, Mass. : Cell Press, 2002 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 39(2021), 3, Seite 407-422.e13 |
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| ISSN Quelle: | 1878-3686 |
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| Abstract: | Diffuse intrinsic pontine glioma (DIPG) is an aggressive childhood tumor of the brainstem with currently no curative treatment available. The vast majority of DIPGs carry a histone H3 mutation leading to a lysine 27-to-methionine exchange (H3K27M). We engineered human induced pluripotent stem cells (iPSCs) to carry an inducible H3.3-K27M allele in the endogenous locus and studied the effects of the mutation in different disease-relevant neural cell types. H3.3-K27M upregulated bivalent promoter-associated developmental genes, producing diverse outcomes in different cell types. While being fatal for iPSCs, H3.3-K27M increased proliferation in neural stem cells (NSCs) and to a lesser extent in oligodendrocyte progenitor cells (OPCs). Only NSCs gave rise to tumors upon induction of H3.3-K27M and TP53 inactivation in an orthotopic xenograft model recapitulating human DIPGs. In NSCs, H3.3-K27M leads to maintained expression of stemness and proliferative genes and a premature activation of OPC programs that together may cause tumor initiation. |
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| DOI: | doi:10.1016/j.ccell.2021.01.005 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.ccell.2021.01.005 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1535610821000490 |
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| | DOI: https://doi.org/10.1016/j.ccell.2021.01.005 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | bivalent chromatin |
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| | DIPG |
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| | glioma |
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| | H3.3-K27M |
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| | H3K27me3 |
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| | H3K4me3 |
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| | iPSC |
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| | NSC |
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| | OPC |
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| | orthotopic xenograft |
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| K10plus-PPN: | 1747449784 |
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| Verknüpfungen: | → Zeitschrift |
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H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model / Haag, Daniel [VerfasserIn]; 4 February 2021 (Online-Ressource)
