Phosphoregulation of HORMA domain protein HIM-3 promotes asymmetric synaptonemal complex disassembly in meiotic prophase in Caenorhabditis elegans

• Verfasst von: Sato-Carlton, Aya [VerfasserIn]
• Verfasst von: Nakamura-Tabuchi, Chihiro [VerfasserIn]
• Verfasst von: Li, Xuan [VerfasserIn]
• Verfasst von: Boog, Hendrik [VerfasserIn]
• Verfasst von: Lehmer, Madison K. [VerfasserIn]
• Verfasst von: Rosenberg, Scott C. [VerfasserIn]
• Verfasst von: Barroso, Consuelo [VerfasserIn]
• Verfasst von: Martinez-Perez, Enrique [VerfasserIn]
• Verfasst von: Corbett, Kevin D. [VerfasserIn]
• Verfasst von: Carlton, Peter Mark [VerfasserIn]
• Titel: Phosphoregulation of HORMA domain protein HIM-3 promotes asymmetric synaptonemal complex disassembly in meiotic prophase in Caenorhabditis elegans
• Verf.angabe: Aya Sato-Carlton, Chihiro Nakamura-Tabuchi, Xuan Li, Hendrik Boog, Madison K. Lehmer, Scott C. Rosenberg, Consuelo Barroso, Enrique Martinez-Perez, Kevin D. Corbett, Peter Mark Carlton
• E-Jahr: 2020
• Jahr: November 11, 2020
• Fussnoten: Gesehen am 05.02.2021
• Titel Quelle: Enthalten in: Public Library of SciencePLoS Genetics
• Ort Quelle: San Francisco, Calif. : Public Library of Science, 2005
• Jahr Quelle: 2020
• Band/Heft Quelle: 16(2020,11) Artikel-Nummer e1008968, 33 Seiten
• ISSN Quelle: 1553-7404
• DOI: doi:10.1371/journal.pgen.1008968
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Caenorhabditis elegans
• Sach-SW: Chromosome staining
• Sach-SW: Gonads
• Sach-SW: Immunostaining
• Sach-SW: Meiosis
• Sach-SW: Oocytes
• Sach-SW: Phosphorylation
• Sach-SW: Synapsis
• K10plus-PPN: 1747632422

Abstract

In the two cell divisions of meiosis, diploid genomes are reduced into complementary haploid sets through the discrete, two-step removal of chromosome cohesion, a task carried out in most eukaryotes by protecting cohesion at the centromere until the second division. In eukaryotes without defined centromeres, however, alternative strategies have been innovated. The best-understood of these is found in the nematode Caenorhabditis elegans: after the single off-center crossover divides the chromosome into two segments, or arms, several chromosome-associated proteins or post-translational modifications become specifically partitioned to either the shorter or longer arm, where they promote the correct timing of cohesion loss through as-yet unknown mechanisms. Here, we investigate the meiotic axis HORMA-domain protein HIM-3 and show that it becomes phosphorylated at its C-terminus, within the conserved “closure motif” region bound by the related HORMA-domain proteins HTP-1 and HTP-2. Binding of HTP-2 is abrogated by phosphorylation of the closure motif in in vitro assays, strongly suggesting that in vivo phosphorylation of HIM-3 likely modulates the hierarchical structure of the chromosome axis. Phosphorylation of HIM-3 only occurs on synapsed chromosomes, and similarly to other previously-described phosphorylated proteins of the synaptonemal complex, becomes restricted to the short arm after designation of crossover sites. Regulation of HIM-3 phosphorylation status is required for timely disassembly of synaptonemal complex central elements from the long arm, and is also required for proper timing of HTP-1 and HTP-2 dissociation from the short arm. Phosphorylation of HIM-3 thus plays a role in establishing the identity of short and long arms, thereby contributing to the robustness of the two-step chromosome segregation.