Identification of HLA class I dependent immunogenic peptides from clonotypic TCRβ expressed in cutaneous T-cell lymphoma

• Verfasst von: Zeng, Jinyang [VerfasserIn]
• Verfasst von: Nguyen, Xuan-Duc [VerfasserIn]
• Verfasst von: Klüter, Harald [VerfasserIn]
• Verfasst von: Schönhaber, Hiltrud [VerfasserIn]
• Verfasst von: Song, Mingxia [VerfasserIn]
• Verfasst von: Schwinn, Nicole [VerfasserIn]
• Verfasst von: Schadendorf, Dirk [VerfasserIn]
• Verfasst von: Goerdt, Sergij [VerfasserIn]
• Verfasst von: Eichmüller, Stefan B. [VerfasserIn]
• Titel: Identification of HLA class I dependent immunogenic peptides from clonotypic TCRβ expressed in cutaneous T-cell lymphoma
• Verf.angabe: Jinyang Zeng, Jan Müller‐Berghaus, Xuan Duc Nguyen, Harald Klüter, Hiltrud Schönhaber, Mingxia Song, Nicole Schwinn, Dirk Schadendorf, Sergij Goerdt, Stefan Eichmüller and Edgar Dippel
• E-Jahr: 2006
• Jahr: 26 September 2006
• Umfang: 5 S.
• Fussnoten: Gesehen am 09.02.2021
• Titel Quelle: Enthalten in: International journal of cancer
• Ort Quelle: Bognor Regis : Wiley-Liss, 1966
• Jahr Quelle: 2006
• Band/Heft Quelle: 119(2006), 10, Seite 2476-2480
• ISSN Quelle: 1097-0215
• DOI: doi:10.1002/ijc.22113
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cutaneous T-cell lymphoma
• Sach-SW: immunotherapy
• Sach-SW: reverse immunology
• Sach-SW: T cells
• K10plus-PPN: 1747879525

Abstract

The clonotypic T-cell receptor (TCR) is a potential target antigen for specific immunotherapy of cutaneous T-cell lymphoma (CTCL). We identified T-cell epitopes from the rearranged TCR β chain of the malignant T-cell population by the “reverse immunology” approach. Peptide-specific T-cell lines were generated against predicted epitopes and tested for the recognition of tumor cells and cells transfected with the full-length DNA coding for TCRV β chain. Two peptides derived from the clonotypic TCRVβ of a HLA-A2 positive patient could induce peptide-specific T cells from peripheral blood mononuclear cells of healthy donors and the patient as assessed by IFN-γ ELISpot assay. Furthermore, the reactive CTLs efficiently recognized autologous Sézary tumor cells, as well as HLA-A2 positive 293 cells transfected with recombinant plasmid expressing the corresponding TCRVβ29 protein. Similar results were obtained in a HLA-A3+ patient for TCRVβ7-Jβ2.7. In conclusion, our experiments show that the TCR β chain harbors epitopes suitable as targets for specific vaccination which might be a promising approach for the specific immunotherapy of cutaneous T-cell lymphoma patients. © 2006 Wiley-Liss, Inc.