Identification of a cross-reactive HLA-DRB1*0301-restricted CD4 T cell response directed against cholesterol-binding cytolysins from two different pathogens

• Verfasst von: Paschen, Annette [VerfasserIn]
• Verfasst von: Song, Mingxia [VerfasserIn]
• Verfasst von: Nguyen, Xuan-Duc [VerfasserIn]
• Verfasst von: Osen, Wolfram [VerfasserIn]
• Verfasst von: Schadendorf, Dirk [VerfasserIn]
• Verfasst von: Geginat, Gernot [VerfasserIn]
• Titel: Identification of a cross-reactive HLA-DRB1*0301-restricted CD4 T cell response directed against cholesterol-binding cytolysins from two different pathogens
• Verf.angabe: Annette Paschen, Mingxia Song, Simone Schenk, Jozef Janda, Xuan Duc Nguyen, Wolfram Osen, Dirk Schadendorf, Gernot Geginat
• E-Jahr: 2006
• Jahr: 30 May 2006
• Umfang: 10 S.
• Fussnoten: Gesehen am 09.02.2021
• Titel Quelle: Enthalten in: Microbes and infection
• Ort Quelle: Paris [u.a.] : Elsevier, 1999
• Jahr Quelle: 2006
• Band/Heft Quelle: 8(2006), 8, Seite 2034-2043
• ISSN Quelle: 1769-714X
• DOI: doi:10.1016/j.micinf.2006.03.010
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Bacterial infection
• Sach-SW: Immune responses
• Sach-SW: T helper cells
• K10plus-PPN: 1747884057

Abstract

Cholesterol-binding cytolysins constitute an evolutionarily conserved family of pore-forming proteins expressed by different Gram-positive pathogens. Listeriolysin O, one well-characterized member of the cytolysin family, is also known to induce specific CD4 and CD8 T cell responses upon infection of mice with Listeria monocytogenes. Here we describe an HLA-DRB1*0301-restricted listeriolysin O-derived T cell epitope that is conserved among several members of the cytolysin family. An HLA-DRB1*0301-restricted CD4+T cell line, established from spleen lymphocytes of L. monocytogenes-infected HLA-DRB1*0301-transgenic mice, cross-reacted with a homologous peptide from perfringolysin O, a cytolysin expressed by Clostridium perfringens. Ex vivo analysis of infected mice revealed an even broader cross-reaction of T cells with homologous peptides derived from perfringolysin O, streptolysin O, and cereolysin O. Interestingly, a cross-reactive memory CD4+T cell response against the homologous peptides derived from listeriolysin O and perfringolysin O could also be detected in the blood from healthy HLA-DRB1*0301+ human donors. Remarkably, this response was even present in donors who did not exhibit a memory T cell reactivity against a second, non-conserved HLA-DRB1*0301-restricted LLO-derived CD4 T cell epitope, suggesting that cytolysin-producing bacteria other than L. monocytogenes can stimulate a cross-reactive cytolysin-specific immunity.