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Verfasst von:Ludwig-Peitsch, Wiebke [VerfasserIn]   i
 Schnölzer, Martina [VerfasserIn]   i
 Spring, Herbert [VerfasserIn]   i
 Franke, Werner [VerfasserIn]   i
Titel:Drebrin is a widespread actin-associating protein enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells
Verf.angabe:Wiebke K. Peitsch, Christine Grund, Caecilia Kuhn, Martina Schnölzer, Herbert Spring, Monika Schmelz, Werner W. Franke
E-Jahr:2012
Jahr:20 February 2012
Jahr des Originals:1999
Umfang:12 S.
Fussnoten:Available online 20 February 2012 ; Elektronische Reproduktion der Druck-Ausgabe ; Gesehen am 11.02.2021
Titel Quelle:Enthalten in: European journal of cell biology
Ort Quelle:München : Elsevier, 1998
Jahr Quelle:1999
Band/Heft Quelle:78(1999), 11, Seite 767-778
ISSN Quelle:1618-1298
Abstract:Using immunoblotting, immunprecipitation with subsequent fragment mass spectrometry, and immunolocalization techniques, we have detected the actin-binding ca. 120-kDa protein drebrin, originally identified in - and thought to be specific for - neuronal cells, in diverse kinds of human and bovine nonneuronal cells. Drebrin has been found in numerous cell culture lines and in many tissues of epithelial, endothelial, smooth muscle and neural origin but not in, for example, cardiac, skeletal and certain types of smooth muscle cells, in hepatocytes and in the human epithelium-derived cell culture line A-431. By double-label fluorescence microscopy we have found drebrin enriched in actin microfilament bundles associated with plaques of cell-cell contact sites representing adhering junctions. These drebrin-positive, adhering junction-associated bundles, however, are not identical with the vinculin-containing, junction-attached bundles, and in the same cell both subtypes of microfilament-anchoring plaques are readily distinguished by immunolocalization comparing drebrin and vinculin. The intracellular distribution of the drebrin- and the vinculin-based microfilament systems has been studied in detail by confocal fluorescence laser scanning microscopy in monolayers of the polar epithelial cell lines, MCF-7 and PLC, and drebrin has been found to be totally and selectively absent in the notoriously vinculin-rich focal adhesions. The occurrence and the possible functions of drebrin in non-neuronal cells, notably epithelial cells, and the significance of the existence of two different actin-anchoring junctional plaques is discussed.
DOI:doi:10.1016/S0171-9335(99)80027-2
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/S0171-9335(99)80027-2
 Volltext: https://www.sciencedirect.com/science/article/pii/S0171933599800272
 DOI: https://doi.org/10.1016/S0171-9335(99)80027-2
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:actin
 adhering junctions
 Drebrin
 microfilaments
 vinculin
K10plus-PPN:1748078968
Verknüpfungen:→ Zeitschrift

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