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Status: Bibliographieeintrag

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Verfasst von:Goeppert, Benjamin [VerfasserIn]   i
 Schmidt, Christopher Roman [VerfasserIn]   i
 Geiselhart, Lea [VerfasserIn]   i
 Dutruel, Céline [VerfasserIn]   i
 Capper, David [VerfasserIn]   i
 Renner, Marcus [VerfasserIn]   i
 Vogel, Monika Nadja [VerfasserIn]   i
 Zachskorn, Cornelia [VerfasserIn]   i
 Zinke, Jenny [VerfasserIn]   i
 Campos, Benito [VerfasserIn]   i
 Schmezer, Peter [VerfasserIn]   i
 Popanda, Odilia [VerfasserIn]   i
 Wick, Wolfgang [VerfasserIn]   i
 Weller, Michael [VerfasserIn]   i
 Meyermann, Richard [VerfasserIn]   i
 Schittenhelm, Jens Florian [VerfasserIn]   i
 Harter, Patrick Nikolaus [VerfasserIn]   i
 Simon, Perikles [VerfasserIn]   i
 Weichert, Wilko [VerfasserIn]   i
 Schirmacher, Peter [VerfasserIn]   i
 Plass, Christoph [VerfasserIn]   i
 Mittelbronn, Michel Guy André [VerfasserIn]   i
Titel:Differential expression of the tumor suppressor A-kinase anchor protein 12 in human diffuse and pilocytic astrocytomas is regulated by promoter methylation
Verf.angabe:Benjamin Goeppert, MD, Christopher R. Schmidt, PhD, Lea Geiselhart, PhD, Céline Dutruel, PhD, David Capper, MD, Marcus Renner, PhD, Monika Nadja Vogel, MD, Cornelia Zachskorn, TA, Jenny Zinke, Benito Campos, MD, Peter Schmezer, PhD, Odilia Popanda, PhD, Wolfgang Wick, MD, Michael Weller, MD, Richard Meyermann, MD, Jens Schittenhelm, MD, Patrick Nikolaus Harter, MD, Perikles Simon, MD, PhD, Wilko Weichert, MD, Peter Schirmacher, MD, Christoph Plass, MD, and Michel Mittelbronn, MD
E-Jahr:2013
Jahr:01 October 2013
Umfang:9 S.
Fussnoten:Gesehen am 11.02.2020
Titel Quelle:Enthalten in: Journal of neuropathology and experimental neurology
Ort Quelle:Oxford : Oxford University Press, 1942
Jahr Quelle:2013
Band/Heft Quelle:72(2013), 10, Seite 933-941
ISSN Quelle:1554-6578
Abstract:The scaffold protein A-kinase anchor protein 12 (AKAP12) exerts tumor suppressor activity and is downregulated in several tumor entities. We characterized AKAP12 expression and regulation in astrocytomas, including pilocytic and diffusely infiltrating astrocytomas. We examined 194 human gliomas and 23 normal brain white matter samples by immunohistochemistry or immunoblotting for AKAP12 expression. We further performed quantitative methylation analysis of the AKAP12 promoter by MassARRAY® of normal brain, World Health Organization (WHO) grade I to IV astrocytomas, and glioma cell lines. Our results show that AKAP12 is expressed in a perivascular distribution in normal CNS, strongly upregulated in tumor cells in pilocytic astrocytomas, and weakly expressed in diffuse astrocytomas of WHO grade II to IV. Methyl-ation analyses revealed specific hypermethylation of AKAP12α promoter in WHO grade II to IV astrocytomas. Restoration experiments using 5-aza-2′-deoxycytidine in primary glioblastoma cells decreased AKAP12α promoter methylation and markedly increased AKAP12α mRNA levels. In summary, we demonstrate that AKAP12 is differentially expressed in human astrocytomas showing high expression in pilocytic but low expression in diffuse astrocytomas of all WHO-grades. Our results further indicate that epigenetic mechanisms are involved in silencing AKAP12 in diffuse astrocytomas; however, a tumor suppressive role of AKAP12 in distinct astrocytoma subtypes remains to be determined.
DOI:doi:10.1097/NEN.0b013e3182a59a88
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1097/NEN.0b013e3182a59a88
 DOI: https://doi.org/10.1097/NEN.0b013e3182a59a88
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:174810425X
Verknüpfungen:→ Zeitschrift

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