| |  Online-Ressource |
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| Verfasst von: | Zila, Vojtech [VerfasserIn]  |
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| | Margiotta, Erica [VerfasserIn] |
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| | Turoňová, Beata [VerfasserIn] |
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| | Müller, Thorsten G. [VerfasserIn] |
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| | Zimmerli, Christian Eugen [VerfasserIn] |
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| | Mattei, Simone [VerfasserIn] |
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| | Allegretti, Matteo [VerfasserIn] |
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| | Börner, Kathleen [VerfasserIn] |
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| | Rada, Jona [VerfasserIn] |
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| | Müller, Barbara [VerfasserIn] |
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| | Lusic, Marina [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| | Beck, Martin [VerfasserIn] |
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| Titel: | Cone-shaped HIV-1 capsids are transported through intact nuclear pores |
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| Verf.angabe: | Vojtech Zila, Erica Margiotta, Beata Turoňová, Thorsten G. Müller, Christian E. Zimmerli, Simone Mattei, Matteo Allegretti, Kathleen Börner, Jona Rada, Barbara Müller, Marina Lusic, Hans-Georg Kräusslich, Martin Beck |
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| E-Jahr: | 2021 |
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| Jahr: | 10 February 2021 |
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| Umfang: | 33 Seiten |
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| Fussnoten: | Gesehen am 12.02.2021 |
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| Titel Quelle: | Enthalten in: Cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1974 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 184(2021), 4, Seite 1032-1046, e1-e18 |
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| ISSN Quelle: | 1097-4172 |
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| Abstract: | Human immunodeficiency virus (HIV-1) remains a major health threat. Viral capsid uncoating and nuclear import of the viral genome are critical for productive infection. The size of the HIV-1 capsid is generally believed to exceed the diameter of the nuclear pore complex (NPC), indicating that capsid uncoating has to occur prior to nuclear import. Here, we combined correlative light and electron microscopy with subtomogram averaging to capture the structural status of reverse transcription-competent HIV-1 complexes in infected T cells. We demonstrated that the diameter of the NPC in cellulo is sufficient for the import of apparently intact, cone-shaped capsids. Subsequent to nuclear import, we detected disrupted and empty capsid fragments, indicating that uncoating of the replication complex occurs by breaking the capsid open, and not by disassembly into individual subunits. Our data directly visualize a key step in HIV-1 replication and enhance our mechanistic understanding of the viral life cycle. |
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| DOI: | doi:10.1016/j.cell.2021.01.025 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.cell.2021.01.025 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0092867421000684 |
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| | DOI: https://doi.org/10.1016/j.cell.2021.01.025 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | capsid |
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| | correlative light and electron microscopy |
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| | cryoelectron tomography |
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| | electron tomography |
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| | human immunodeficiency virus |
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| | nuclear import |
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| | nuclear pore complex |
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| | uncoating |
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| K10plus-PPN: | 1748141732 |
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| Verknüpfungen: | → Zeitschrift |
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Cone-shaped HIV-1 capsids are transported through intact nuclear pores / Zila, Vojtech [VerfasserIn]; 10 February 2021 (Online-Ressource)
