| Online-Ressource |
Verfasst von: | Vajda, Flóra [VerfasserIn]  |
| Jordi, N. [VerfasserIn]  |
| Dalkara, D. [VerfasserIn]  |
| Joly, S. [VerfasserIn]  |
| Christ, F. [VerfasserIn]  |
| Tews, Björn [VerfasserIn]  |
| Schwab, M. E. [VerfasserIn]  |
| Pernet, V. [VerfasserIn]  |
Titel: | Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve |
Verf.angabe: | F. Vajda, N. Jordi, D. Dalkara, S. Joly, F. Christ, B. Tews, M.E. Schwab and V. Pernet |
Jahr: | 2015 |
Jahr des Originals: | 2014 |
Umfang: | 13 S. |
Fussnoten: | published online 26 September 2014 ; Gesehen am 12.02.2021 |
Titel Quelle: | Enthalten in: Cell death and differentiation |
Ort Quelle: | Houndmills, Basingstoke : Nature Publishing Group, 1997 |
Jahr Quelle: | 2015 |
Band/Heft Quelle: | 22(2015), 2, Seite 323-335 |
ISSN Quelle: | 1476-5403 |
Abstract: | Nogo-A is a well-known myelin-enriched inhibitory protein for axonal growth and regeneration in the central nervous system (CNS). Besides oligodendrocytes, our previous data revealed that Nogo-A is also expressed in subpopulations of neurons including retinal ganglion cells, in which it can have a positive role in the neuronal growth response after injury, through an unclear mechanism. In the present study, we analyzed the opposite roles of glial versus neuronal Nogo-A in the injured visual system. To this aim, we created oligodendrocyte (Cnp-Cre+/−xRtn4/Nogo-Aflox/flox) and neuron-specific (Thy1-Cretg+xRtn4flox/flox) conditional Nogo-A knock-out (KO) mouse lines. Following complete intraorbital optic nerve crush, both spontaneous and inflammation-mediated axonal outgrowth was increased in the optic nerves of the glia-specific Nogo-A KO mice. In contrast, neuron-specific deletion of Nogo-A in a KO mouse line or after acute gene recombination in retinal ganglion cells mediated by adeno-associated virus serotype 2.Cre virus injection in Rtn4flox/flox animals decreased axon sprouting in the injured optic nerve. These results therefore show that selective ablation of Nogo-A in oligodendrocytes and myelin in the optic nerve is more effective at enhancing regrowth of injured axons than what has previously been observed in conventional, complete Nogo-A KO mice. Our data also suggest that neuronal Nogo-A in retinal ganglion cells could participate in enhancing axonal sprouting, possibly by cis-interaction with Nogo receptors at the cell membrane that may counteract trans-Nogo-A signaling. We propose that inactivating Nogo-A in glia while preserving neuronal Nogo-A expression may be a successful strategy to promote axonal regeneration in the CNS. |
DOI: | doi:10.1038/cdd.2014.147 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/cdd.2014.147 |
| Volltext: https://www.nature.com/articles/cdd2014147 |
| DOI: https://doi.org/10.1038/cdd.2014.147 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1748199080 |
Verknüpfungen: | → Zeitschrift |
Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve / Vajda, Flóra [VerfasserIn]; 2015 (Online-Ressource)