| |  Online-Ressource |
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| Verfasst von: | Hartmann, Kristina [VerfasserIn]  |
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| | Corvey, Carsten [VerfasserIn] |
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| | Skerka, Christine [VerfasserIn] |
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| | Kirschfink, Michael [VerfasserIn] |
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| | Karas, Michael [VerfasserIn] |
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| | Brade, Volker [VerfasserIn] |
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| | Miller, Jennifer C. [VerfasserIn] |
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| | Stevenson, Brian [VerfasserIn] |
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| | Wallich, Reinhard [VerfasserIn] |
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| | Zipfel, Peter F. [VerfasserIn] |
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| | Kraiczy, Peter [VerfasserIn] |
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| Titel: | Functional characterization of BbCRASP-2, a distinct outer membrane protein of Borrelia burgdorferi that binds host complement regulators factor H and FHL-1 |
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| Verf.angabe: | Kristina Hartmann, Carsten Corvey, Christine Skerka, Michael Kirschfink, Michael Karas, Volker Brade, Jennifer C. Miller, Brian Stevenson, Reinhard Wallich, Peter F. Zipfel and Peter Kraiczy |
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| E-Jahr: | 2006 |
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| Jahr: | 01 August 2006 |
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| Umfang: | 17 S. |
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| Fussnoten: | Gesehen am 15.02.2021 |
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| Titel Quelle: | Enthalten in: Molecular microbiology |
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| Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1987 |
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| Jahr Quelle: | 2006 |
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| Band/Heft Quelle: | 61(2006), 5, Seite 1220-1236 |
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| ISSN Quelle: | 1365-2958 |
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| Abstract: | Borrelia burgdorferi, the aetiological agent of Lyme disease, employs sophisticated means to survive in diverse mammalian hosts. Recent studies demonstrated that acquisition of complement regulators factor H and factor H-like protein-1 (FHL-1) allows spirochetes to resist complement-mediated killing. Serum-resistant B. burgdorferi express up to five distinct complement regulator-acquiring surface proteins (CRASPs) that bind factor H and/or FHL-1. In this study we have identified and characterized one of those B. burgdorferi proteins, named BbCRASP-2. BbCRASP-2 is distinct from the four previously identified factor H/FHL-1-binding CRASPs of B. burgdorferi strains. The single copy of the gene encoding BbCRASP-2, cspZ, is located on the linear plasmid lp28-3. BbCRASP-2 is highly divergent from the factor H/FHL-1-binding protein BbCRASP-1 and from members of the factor H-binding Erp (OspE/F-related) protein family. Peptide mapping analysis revealed that the factor H/FHL-1 binding site is discontinuous and it was found that C-terminal truncations abrogate factor H and FHL-1 binding. The predominant BbCRASP-2 binding site of both host complement regulators was mapped to the short consensus repeat 7 (SCR 7). Factor H and FHL-1 bound to BbCRASP-2 maintain cofactor activity for factor I-mediated C3b inactivation and accelerate the decay of the C3 convertase. Expression of BbCRASP-2 in serum-sensitive B. burgdorferi mutant B313 increased resistance to complement-mediated lysis. The characterization of BbCRASP-2 now provides a complete picture of the three diverse complement regulator-binding protein families of B. burgdorferi yielding new insights into the pathogenesis of Lyme disease. |
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| DOI: | doi:10.1111/j.1365-2958.2006.05318.x |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1111/j.1365-2958.2006.05318.x |
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| | Volltext: https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05318.x |
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| | DOI: https://doi.org/10.1111/j.1365-2958.2006.05318.x |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Amino Acid Sequence |
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| | Animals |
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| | Bacterial Proteins |
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| | Blotting, Western |
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| | Borrelia burgdorferi |
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| | Complement Factor H |
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| | Electrophoresis, Polyacrylamide Gel |
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| | Humans |
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| | Intracellular Signaling Peptides and Proteins |
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| | LIM Domain Proteins |
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| | Lyme Disease |
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| | Mass Spectrometry |
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| | Membrane Proteins |
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| | Mice |
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| | Mice, Inbred BALB C |
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| | Microscopy, Fluorescence |
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| | Molecular Sequence Data |
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| | Muscle Proteins |
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| | Protein Binding |
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| | Surface Plasmon Resonance |
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| K10plus-PPN: | 1748290371 |
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| Verknüpfungen: | → Zeitschrift |
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Functional characterization of BbCRASP-2, a distinct outer membrane protein of Borrelia burgdorferi that binds host complement regulators factor H and FHL-1 / Hartmann, Kristina [VerfasserIn]; 01 August 2006 (Online-Ressource)
