| |  Online-Ressource |
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| Verfasst von: | Lehmann, Thorsten G. [VerfasserIn]  |
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| | Koeppel, Thomas [VerfasserIn] |
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| | Münch, Steffen [VerfasserIn] |
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| | Heger, Michael [VerfasserIn] |
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| | Kirschfink, Michael [VerfasserIn] |
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| | Klar, Ernst [VerfasserIn] |
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| | Post, Stefan [VerfasserIn] |
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| Titel: | Impact of inhibition of complement by sCR1 on hepatic microcirculation after warm ischemia |
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| Verf.angabe: | T.G. Lehmann, T.A. Koeppel, S. Münch, M. Heger, M. Kirschfink, E. Klar, S. Post |
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| E-Jahr: | 2001 |
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| Jahr: | 25 May 2002 |
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| Jahr des Originals: | 2001 |
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| Umfang: | 9 S. |
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| Fussnoten: | Gesehen am 17.02.2021 |
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| Titel Quelle: | Enthalten in: Microvascular research |
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| Ort Quelle: | Orlando, Fla. : Academic Press, 1968 |
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| Jahr Quelle: | 2001 |
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| Band/Heft Quelle: | 62(2001), 3, Seite 284-292 |
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| ISSN Quelle: | 1095-9319 |
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| Abstract: | Recent observations provide evidence that complement is implicated as an important factor in the pathophysiology of ischemia/reperfusion injury (IRI). Here, we assessed the effects of complement inhibition on hepatic microcirculation by in vivo microscopy (IVM) using a rat model of warm hepatic ischemia clamping the left pedicle for 70 min. Ten animals received the physiological complement regulator soluble complement receptor type 1 (sCR1) intravenously 1 min prior to reperfusion. Controls were given an equal amount of Ringer's solution (n = 10). Microvascular perfusion and leukocyte adhesion were studied 30 to 100 min after reperfusion by IVM. Microvascular perfusion in hepatic sinusoids was significantly improved in the sCR1 group (80.6 +/- 0.6% of all observed sinusoids were perfused [sCR1] vs 67.3 +/- 1.2% [controls]). The number of adherent leukocytes was reduced in sinusoids (49.9 +/- 3.4 [sCR1] vs 312.3 +/- 14.2 in controls [adherent leukocytes per square millimeter of liver surface]; P < 0.001) as well as in postsinusoidal venules after sCR1 treatment (230.9 +/- 21.7 [sCR1] vs 1906.5 +/- 93.5 [controls] [adherent leukocytes per square millimeter of endothelial surface]; P < 0.001). Reflecting reduced hepatocyte injury, liver transaminases were decreased significantly upon sCR1 treatment compared to controls. Our results provide further evidence that complement plays a decisive role in warm hepatic IRI. Therefore, we conclude that complement inhibition by sCR1 is effective as a therapeutical approach to reduce microcirculatory disorders after reperfusion following warm organ ischemia. |
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| DOI: | doi:10.1006/mvre.2001.2342 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1006/mvre.2001.2342 |
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| | Volltext: https://www.sciencedirect.com/science/article/abs/pii/S0026286201923428 |
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| | DOI: https://doi.org/10.1006/mvre.2001.2342 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Cell Adhesion |
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| | Complement C1 Inactivator Proteins |
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| | Complement C1s |
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| | Endothelium, Vascular |
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| | Hemodynamics |
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| | Injections, Intravenous |
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| | Ischemia |
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| | Laser-Doppler Flowmetry |
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| | Leukocytes |
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| | Liver Circulation |
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| | Male |
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| | Microcirculation |
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| | Microscopy, Fluorescence |
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| | Rats |
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| | Rats, Wistar |
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| | Receptors, Complement |
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| | Reperfusion Injury |
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| | Temperature |
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| | Time Factors |
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| | Venules |
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| | Video Recording |
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| K10plus-PPN: | 1748479733 |
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| Verknüpfungen: | → Zeitschrift |
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Impact of inhibition of complement by sCR1 on hepatic microcirculation after warm ischemia / Lehmann, Thorsten G. [VerfasserIn]; 25 May 2002 (Online-Ressource)
