| Online-Ressource |
Verfasst von: | Zavrski, Ivana [VerfasserIn]  |
| Naujokat, Cord [VerfasserIn]  |
| Niemöller, Kathrin [VerfasserIn]  |
| Jakob, Christian [VerfasserIn]  |
| Heider, Ulrike [VerfasserIn]  |
| Langelotz, Corinna [VerfasserIn]  |
| Fleissner, Claudia [VerfasserIn]  |
| Eucker, Jan [VerfasserIn]  |
| Possinger, Kurt [VerfasserIn]  |
| Sezer, Orhan [VerfasserIn]  |
Titel: | Proteasome inhibitors induce growth inhibition and apoptosis in myeloma cell lines and in human bone marrow myeloma cells irrespective of chromosome 13 deletion |
Verf.angabe: | Ivana Zavrski, Cord Naujokat, Kathrin Niemöller, Christian Jakob, Ulrike Heider, Corinna Langelotz, Claudia Fleissner, Jan Eucker, Kurt Possinger & Orhan Sezer |
Jahr: | 2003 |
Umfang: | 9 S. |
Fussnoten: | Gesehen am 19.02.2021 |
Titel Quelle: | Enthalten in: Journal of cancer research and clinical oncology |
Ort Quelle: | Berlin : Springer, 1904 |
Jahr Quelle: | 2003 |
Band/Heft Quelle: | 129(2003), 7, Seite 383-391 |
ISSN Quelle: | 1432-1335 |
Abstract: | PURPOSE: In this study, we investigated the effects of cell-permeable proteasome inhibitors MG-132, MG-262, PSI, and lactacystin on multiple myeloma cell lines OPM-2, U266, RPMI 8226-S, freshly isolated plasma cells with or without deletion of chromosome 13 from patients with multiple myeloma and plasma cell leukemia, and CD34+ human hematopoietic stem cells. The effects of proteasome inhibitors on cell cycle progression, cell growth, and apoptosis were determined. METHODS: MTT-assay was used to examine the cytotoxicity, and annexin-V staining to quantify apoptosis. Cell cycle analyses were performed using 7-ADD and Ki-67 staining by flow cytometry. RESULTS: PSI was the most potent proteasome inhibitor among those tested with a half maximal cytotoxicity (IC(50)) of 5.7 nM, followed by MG-262, MG-132, and lactacystin. Growth inhibition occurred irrespective of chromosome 13 status. Cell cycle arrest occurred in a dose- and time-dependent manner. Low, subapoptotic dosages led to a partial loss of Ki-67 antigen, whereas apoptotic dosages led to reduced Ki-67 levels. Apoptosis was partially dependent on activation of caspase-3, since Ac-DEVD-cho, a caspase-3 inhibitor, could reduce apoptosis significantly. The cytotoxicity of the four proteasome inhibitors tested was significantly lower in human hematopoietic stem cells than in myeloma cells. CONCLUSIONS: Our results show that proteasome inhibitors induce time- and dose-dependent cell cycle alterations, growth inhibition, and apoptosis in human myeloma cells irrespective of chromosome 13 deletion. |
DOI: | doi:10.1007/s00432-003-0454-6 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s00432-003-0454-6 |
| DOI: https://doi.org/10.1007/s00432-003-0454-6 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Antigens, CD34 |
| Apoptosis |
| Caspase 3 |
| Caspase 7 |
| Caspases |
| Cell Cycle |
| Cell Division |
| Chromosome Deletion |
| Chromosomes, Human, Pair 13 |
| Cysteine Endopeptidases |
| Hematopoietic Stem Cells |
| Humans |
| Multienzyme Complexes |
| Multiple Myeloma |
| Proteasome Endopeptidase Complex |
| Tumor Cells, Cultured |
K10plus-PPN: | 1748665960 |
Verknüpfungen: | → Zeitschrift |
Proteasome inhibitors induce growth inhibition and apoptosis in myeloma cell lines and in human bone marrow myeloma cells irrespective of chromosome 13 deletion / Zavrski, Ivana [VerfasserIn]; 2003 (Online-Ressource)