Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Zavrski, Ivana [VerfasserIn]   i
 Naujokat, Cord [VerfasserIn]   i
 Niemöller, Kathrin [VerfasserIn]   i
 Jakob, Christian [VerfasserIn]   i
 Heider, Ulrike [VerfasserIn]   i
 Langelotz, Corinna [VerfasserIn]   i
 Fleissner, Claudia [VerfasserIn]   i
 Eucker, Jan [VerfasserIn]   i
 Possinger, Kurt [VerfasserIn]   i
 Sezer, Orhan [VerfasserIn]   i
Titel:Proteasome inhibitors induce growth inhibition and apoptosis in myeloma cell lines and in human bone marrow myeloma cells irrespective of chromosome 13 deletion
Verf.angabe:Ivana Zavrski, Cord Naujokat, Kathrin Niemöller, Christian Jakob, Ulrike Heider, Corinna Langelotz, Claudia Fleissner, Jan Eucker, Kurt Possinger & Orhan Sezer
Jahr:2003
Umfang:9 S.
Fussnoten:Gesehen am 19.02.2021
Titel Quelle:Enthalten in: Journal of cancer research and clinical oncology
Ort Quelle:Berlin : Springer, 1904
Jahr Quelle:2003
Band/Heft Quelle:129(2003), 7, Seite 383-391
ISSN Quelle:1432-1335
Abstract:PURPOSE: In this study, we investigated the effects of cell-permeable proteasome inhibitors MG-132, MG-262, PSI, and lactacystin on multiple myeloma cell lines OPM-2, U266, RPMI 8226-S, freshly isolated plasma cells with or without deletion of chromosome 13 from patients with multiple myeloma and plasma cell leukemia, and CD34+ human hematopoietic stem cells. The effects of proteasome inhibitors on cell cycle progression, cell growth, and apoptosis were determined. METHODS: MTT-assay was used to examine the cytotoxicity, and annexin-V staining to quantify apoptosis. Cell cycle analyses were performed using 7-ADD and Ki-67 staining by flow cytometry. RESULTS: PSI was the most potent proteasome inhibitor among those tested with a half maximal cytotoxicity (IC(50)) of 5.7 nM, followed by MG-262, MG-132, and lactacystin. Growth inhibition occurred irrespective of chromosome 13 status. Cell cycle arrest occurred in a dose- and time-dependent manner. Low, subapoptotic dosages led to a partial loss of Ki-67 antigen, whereas apoptotic dosages led to reduced Ki-67 levels. Apoptosis was partially dependent on activation of caspase-3, since Ac-DEVD-cho, a caspase-3 inhibitor, could reduce apoptosis significantly. The cytotoxicity of the four proteasome inhibitors tested was significantly lower in human hematopoietic stem cells than in myeloma cells. CONCLUSIONS: Our results show that proteasome inhibitors induce time- and dose-dependent cell cycle alterations, growth inhibition, and apoptosis in human myeloma cells irrespective of chromosome 13 deletion.
DOI:doi:10.1007/s00432-003-0454-6
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1007/s00432-003-0454-6
 DOI: https://doi.org/10.1007/s00432-003-0454-6
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Antigens, CD34
 Apoptosis
 Caspase 3
 Caspase 7
 Caspases
 Cell Cycle
 Cell Division
 Chromosome Deletion
 Chromosomes, Human, Pair 13
 Cysteine Endopeptidases
 Hematopoietic Stem Cells
 Humans
 Multienzyme Complexes
 Multiple Myeloma
 Proteasome Endopeptidase Complex
 Tumor Cells, Cultured
K10plus-PPN:1748665960
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68701398   QR-Code
zum Seitenanfang