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| Verfasst von: | Zavrski, Ivana [VerfasserIn]  |
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| | Naujokat, Cord [VerfasserIn] |
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| | Niemöller, Kathrin [VerfasserIn] |
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| | Jakob, Christian [VerfasserIn] |
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| | Heider, Ulrike [VerfasserIn] |
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| | Langelotz, Corinna [VerfasserIn] |
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| | Fleissner, Claudia [VerfasserIn] |
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| | Eucker, Jan [VerfasserIn] |
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| | Possinger, Kurt [VerfasserIn] |
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| | Sezer, Orhan [VerfasserIn] |
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| Titel: | Proteasome inhibitors induce growth inhibition and apoptosis in myeloma cell lines and in human bone marrow myeloma cells irrespective of chromosome 13 deletion |
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| Verf.angabe: | Ivana Zavrski, Cord Naujokat, Kathrin Niemöller, Christian Jakob, Ulrike Heider, Corinna Langelotz, Claudia Fleissner, Jan Eucker, Kurt Possinger & Orhan Sezer |
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| Jahr: | 2003 |
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| Umfang: | 9 S. |
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| Fussnoten: | Gesehen am 19.02.2021 |
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| Titel Quelle: | Enthalten in: Journal of cancer research and clinical oncology |
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| Ort Quelle: | Berlin : Springer, 1904 |
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| Jahr Quelle: | 2003 |
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| Band/Heft Quelle: | 129(2003), 7, Seite 383-391 |
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| ISSN Quelle: | 1432-1335 |
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| Abstract: | PURPOSE: In this study, we investigated the effects of cell-permeable proteasome inhibitors MG-132, MG-262, PSI, and lactacystin on multiple myeloma cell lines OPM-2, U266, RPMI 8226-S, freshly isolated plasma cells with or without deletion of chromosome 13 from patients with multiple myeloma and plasma cell leukemia, and CD34+ human hematopoietic stem cells. The effects of proteasome inhibitors on cell cycle progression, cell growth, and apoptosis were determined. METHODS: MTT-assay was used to examine the cytotoxicity, and annexin-V staining to quantify apoptosis. Cell cycle analyses were performed using 7-ADD and Ki-67 staining by flow cytometry. RESULTS: PSI was the most potent proteasome inhibitor among those tested with a half maximal cytotoxicity (IC(50)) of 5.7 nM, followed by MG-262, MG-132, and lactacystin. Growth inhibition occurred irrespective of chromosome 13 status. Cell cycle arrest occurred in a dose- and time-dependent manner. Low, subapoptotic dosages led to a partial loss of Ki-67 antigen, whereas apoptotic dosages led to reduced Ki-67 levels. Apoptosis was partially dependent on activation of caspase-3, since Ac-DEVD-cho, a caspase-3 inhibitor, could reduce apoptosis significantly. The cytotoxicity of the four proteasome inhibitors tested was significantly lower in human hematopoietic stem cells than in myeloma cells. CONCLUSIONS: Our results show that proteasome inhibitors induce time- and dose-dependent cell cycle alterations, growth inhibition, and apoptosis in human myeloma cells irrespective of chromosome 13 deletion. |
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| DOI: | doi:10.1007/s00432-003-0454-6 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s00432-003-0454-6 |
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| | DOI: https://doi.org/10.1007/s00432-003-0454-6 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Antigens, CD34 |
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| | Apoptosis |
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| | Caspase 3 |
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| | Caspase 7 |
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| | Caspases |
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| | Cell Cycle |
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| | Cell Division |
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| | Chromosome Deletion |
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| | Chromosomes, Human, Pair 13 |
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| | Cysteine Endopeptidases |
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| | Hematopoietic Stem Cells |
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| | Humans |
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| | Multienzyme Complexes |
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| | Multiple Myeloma |
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| | Proteasome Endopeptidase Complex |
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| | Tumor Cells, Cultured |
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| K10plus-PPN: | 1748665960 |
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| Verknüpfungen: | → Zeitschrift |
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Proteasome inhibitors induce growth inhibition and apoptosis in myeloma cell lines and in human bone marrow myeloma cells irrespective of chromosome 13 deletion / Zavrski, Ivana [VerfasserIn]; 2003 (Online-Ressource)
