CD4 depletion in HIV-infected haemophilia patients is associated with rapid clearance of immune complex-coated CD4+ lymphocytes

• Verfasst von: Daniel, Volker [VerfasserIn]
• Verfasst von: Melk, Anette [VerfasserIn]
• Verfasst von: Süsal, Caner [VerfasserIn]
• Verfasst von: Weimer, Rolf [VerfasserIn]
• Verfasst von: Zimmermann, R. [VerfasserIn]
• Verfasst von: Huth-Kühne, A. [VerfasserIn]
• Verfasst von: Opelz, Gerhard [VerfasserIn]
• Titel: CD4 depletion in HIV-infected haemophilia patients is associated with rapid clearance of immune complex-coated CD4+ lymphocytes
• Verf.angabe: V. Daniel, A. Melk, C. Süsal, R. Weimer, R. Zimmermann, A. Huth-Kühne & G. Opelz
• Jahr: 1999
• Umfang: 8 S.
• Fussnoten: Im Titel + hochgestellt ; Gesehen am 19.02.2021
• Titel Quelle: Enthalten in: Clinical & experimental immunology
• Ort Quelle: Oxford : Wiley-Blackwell, 1966
• Jahr Quelle: 1999
• Band/Heft Quelle: 115(1999), 3, Seite 477-484
• ISSN Quelle: 1365-2249
• DOI: doi:10.1046/j.1365-2249.1999.00848.x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Antigen-Antibody Complex
• Sach-SW: Case-Control Studies
• Sach-SW: CD4 Antigens
• Sach-SW: CD4 Lymphocyte Count
• Sach-SW: CD4-Positive T-Lymphocytes
• Sach-SW: Hemophilia A
• Sach-SW: HIV Infections
• Sach-SW: HIV Seronegativity
• Sach-SW: Humans
• Sach-SW: Lymphopenia
• Sach-SW: Male
• Sach-SW: Time Factors
• Sach-SW: Viremia
• K10plus-PPN: 1748720805

Abstract

The predominant immunological finding in HIV+ haemophilia patients is a decrease of CD4+ lymphocytes during progression of the disease. Depletion of CD4+ lymphocytes is paralleled by an increase in the proportion of immune complex-coated CD4+ cells. We examined the hypothesis that the formation of immune complexes on CD4+ lymphocytes is followed by rapid clearance of immune complex-coated CD4+ lymphocytes from the circulation. In this study, the relationship of relative to absolute numbers of immune complex-loaded CD4+ blood lymphocytes and their association with viral load were studied. Two measurements of relative and absolute numbers of gp120-, IgG- and/or IgM-loaded CD4+ lymphocytes were analysed in HIV+ and HIV- haemophilia patients, with a median interval of approx. 3 years. Immune complexes on CD4+ lymphocytes were determined using double-fluorescence flow cytometry and whole blood samples. Viral load was assessed using NASBA and Nuclisens kits. Whereas the proportion of immune complex-coated CD4+ lymphocytes increased with progression of the disease, absolute numbers of immune complex-coated CD4+ lymphocytes in the blood were consistently low. Relative increases of immune complex-coated CD4+ blood lymphocytes were significantly associated with decreases of absolute numbers of circulating CD4+ lymphocytes. The gp120 load on CD4+ blood lymphocytes increased in parallel with the viral load in the blood. These results indicate that immune complex-coated CD4+ lymphocytes are rapidly cleared from the circulation, suggesting that CD4+ reactive autoantibodies and immune complexes are relevant factors in the pathogenesis of AIDS. Relative increases of immune complex-positive cells seem to be a consequence of both an increasing retroviral activity as well as a stronger loading with immune complexes of the reduced number of CD4+ cells remaining during the process of CD4 depletion. The two mechanisms seem to enhance each other and contribute to the progressive CD4 decrease during the course of the disease.